I am already using BL21(DE3)pLysS cells but I am still getting leaky expression in pre-expression samples before IPTG is added. LB broth is tryptone, salt and yeast.
Thanks :)
Hi there,
2 to 5% is the usual range (ie. 20 to 50g/L).
Add to 0.2% glucose.
Glucose has to be filtered sterilized not autoclaved.
If this does not work, you may want to reduced the plasmid copy number by introducing the pncB mutation (described by Jon Beckwith).
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