Hello Fozia Ibrahim
You may use French pressure cell press, also called French press. It is a piece of equipment used to disrupt cell walls and cell membranes. The French press consists of a hydraulic pump that drives a piston. The piston forces the liquid sample through a tiny valve under high pressure. As the sample passes through the valve, the cells experience shear stress, resulting in cellular disruption. Also, as the cells move through the valve, they experience decompression and subsequently expand and rupture.
Another method that you could use is bead beating. Glass or ceramic beads can crack open cells. This kind of mechanical shear is gentle enough to keep organelles intact. You just add beads to a suitable amount of cell suspension and vortex.
The freeze–thaw cell lysis method is a method which you could also try. It creates ice crystals (water expands when it freezes) that melt when the sample is thawed. Several freeze–thaw cycles ultimately rupture the cells. It is comparatively gentle and doesn’t generate any heat. But it’s time-consuming. You may speed up things by moving sample from liquid nitrogen (or an ethanol/dry ice bath) to hot water, just long enough to thaw, then freezing again. Repeat many times (say 8-10 times).
Best.
French press, including Microfluidizer or any other mechanical high-pressure lyser, also glass beads (bead beating), manually for small volumes or with the help of a machine (BeadBeater). Some people use nitrogen grinding too, after freezing the cells in liquid nitrogen. Also, heating up the cells (like a colony PCR) may be enough to lyse them and get their DNA
Depending upon your purposes I found that incubating yeast cells in lithium acetate/SDS solution and then precipitating with ethanol works quite well, at least for PCR. The detailed protocol could be found here.
https://www.protocols.io/view/lithium-acetate-sds-extraction-of-genomic-dna-from-6qpvrdq1zgmk/v1