primers are short oligonucleotides specifically designed for sequencing and PCR. But if we don't know where to start sequencing for a novel virus's genome then how the primers can be designed? or if we design a primer by prediction or hypothesis then is there any possibility that it will amplify or sequence the genome in a right way?
Archit Gupta
In these cases you can maybe opt to go for non-synthesis sequencing techniques like Oxford nanopore.
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Robert Adolf Brinzer
Usually sequencing libraries are generated by ligating adapters of a known sequence onto the ends of the DNA fragments.
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Sibnarayan Datta
You can go through the following review article and some of the previous reference listed there.
Article Next-generation sequencing in clinical virology: Discovery o...
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