12 Questions 17 Answers 0 Followers
Questions related from Bipasha Bhattacharjee
I have been having a strange issue growing AH109 and using it for transformation. Usually, I would plate it in YPDA and culture it at YPDA too, the generic protocol that is used for a two-hybrid...
17 August 2021 5,305 4 View
Hi all, I have been using YPDA from some time now to grow yeast, but I haven't been getting yeast growth in 3-4 days. The colonies that appear are tiny and when I am doing head to head interaction...
07 June 2021 5,475 1 View
I have two arabidopsis mutants of SALK and Wisconsin Lox. I genotyped them with LP-RP primers and the LBB and P745 primers to check the homozygous lines. When I've isolated the RNA from it and...
30 May 2021 2,610 6 View
I am transforming yeast grown on YPDA media and then plating them on SD/-Trp/-Leu. After obtaining the transformants, I dilute the colonies in water and plate on SD/-Trp/-Leu/-His /-Ade X-@ Gal...
11 May 2021 6,752 1 View
So, I have been cloning a lot of genes over the years in various plant transformation and overexpression vectors and even though its a struggle always to generate positive clones, I usually am...
27 August 2020 8,562 9 View
Hi all, I need an info on the CABS flex and how it works for protein protein interaction simulations. I have docked structures obtained using cluspro and would like to subject them to MD. Please...
25 April 2020 1,696 1 View
Dear all, I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I...
30 January 2020 9,780 3 View
Dear all, I have been trying to use a pgr106 vector for cloning work. However I am facing many issues when I am trying to digest it in the MCS region. The sites I have selected for cloning is cla1...
30 October 2019 8,482 4 View
Hi all! I've been working with Arabidopsis plants from the past 2 years, however this is the first time I've noticed something strange. My plates containing T2 seeds have a white fungal...
06 June 2019 6,676 9 View
Hello all! So currently I am working on a plant transcription factor SUBUNIT which on overexpression in my model plant, is showing dynamic regulation in qRT PCR on infection with a pathogen as...
26 March 2019 9,010 3 View
Could anyone tell me an alternative to Ligplot+ software which could be used to determine the amino acids and the types of bonding between the interacting amino acids in protein protein...
14 November 2018 7,616 14 View
I have two constructs of arabidopsis genes cloned in pet28 vector, transformed in rosetta. The glycerol stocks made three years back were streaked in kan plates, single colony checked with pcr and...
01 January 1970 9,963 3 View
