Hi,
We are planning to do a pyrosequencing run for transcriptomic analysis of a bacterium, and I am really hoping that we'll also catch some sRNA's, but I am not very optimistic, since these are really short -20-30 bases- and I don't even know if they'll be reverse transcribed or sequenced... Does anyone have an idea?
Hi...
Transcriptome sequencing involves sequencing of long reads generally 75 bases from both ends in a paired end run.
Transcriptome sequencing and small RNA sequencing are very different from each other. During genomic DNA library preparation gDNA was first sheared and than size fractionated on agarose gel and a band of 300 to 600 bases was cut which is then reverese transcribed after adding sequencing specific adapters.
As sRNas are of short length there are no chances that a tarnscriptome run will catch some sRNAs however you may get some precursors of small RNAs.
Hope this info will be usefull...
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