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Hi, I have been overexpressing a gene in the cells, and I have found that the genes could promote cell metabolism. Curiously, the cell morphology enlarges. How can I explain this phenomenon? What...
10 May 2024 4,331 2 View
Can someone help me, I don't know how to show the differences between the output and input voltages on the graph. I have two voltages at the input: Vin1 and Vin2, at the output I have Vout1 and...
10 May 2024 3,261 0 View
For my thesis i'm doing a mediation analysis. There are three predictors (autonomy, job demand and social support). I also test a few interaction-effects. I use three background confounders, based...
09 May 2024 2,097 2 View
I have a graph show the heat flux applied on the heated wall below (Heat flux through X direction). The heat flux can be write as a function of Z (for example q= Z*Z + 1000). Can anyone help me...
08 May 2024 1,624 6 View
What is the role of interprofessional collaboration in optimizing outcomes with ivabradine therapy?Ivabradine Therapy in Heart Failure Management: Optimizing O...
05 May 2024 9,589 2 View
How does ivabradine compare to other pharmacological agents used in heart failure management?Ivabradine Therapy in Heart Failure Management: Optimizing O...
05 May 2024 9,811 4 View
I am trying to extract RNA from bacteriophage MS2 filtrate of 10 log pfu/ml (propagated in E. coli C-3000 host) using Trizol and Qiamp viral RNA extraction kit. The RNA concentration was around...
03 May 2024 3,252 2 View
What role did gravity play in the cosmic web and the formation of galaxies and what role does gravity play in the formation of the solar system?
03 May 2024 3,770 3 View
Hi there, I'm rather ashamed to ask this question but need re-assurance and advice for making dilutions of my primers for a 5'RACE PCR. I am trying to amplify heavy and light chain antibody...
01 May 2024 7,963 0 View
Hi im on my final project for my thesis and i get stucked in cloning phase. My goals is to inserting one gene to vector who has another gene too. So i have a gene called llm2-mutan and llm1 who's...
01 May 2024 4,555 4 View
Hello, I have problems with PCR fragment sequencing (Sanger sequencing on SeqStudio). We perform sequencing reaction with BigDye™ Terminator v3.1 with subsequent purification with BigDye™...
30 April 2024 4,628 3 View
We are currently developing a BFT system. It is necessary to measure the C:N ratio level in BFT. Imhoff cone techniques are used only to observe floc density. However we prefer to measure the C:N...
30 April 2024 4,088 2 View
Dear Sir/Madam, I hope that this email finds you well. My name on the paper entitled "Fertility Table and Thermal Requirements for the Development of Callosobruchus maculatus (F) Reared on...
28 April 2024 2,531 1 View
Where could I buy a magnetic shield layers which suppresses the magnetic field below 1μG?
27 April 2024 1,642 0 View
This question needed to be answered to a civil engineering student studying his first year right now.
23 April 2024 9,162 2 View
What requirements (or qualities) are most important to be a good university professor?
23 April 2024 3,047 5 View
i had come to know that the journals of MDPI is not good for phd publications? please give your opinion. Publishing with MDPI have good impact on phd research or not
22 April 2024 7,594 1 View
need answer in a tleast 20 lines
22 April 2024 5,503 0 View
Is it possible to balance environmental protection with economic benefits andenvironmental impacts of economic growth in India?
21 April 2024 4,980 8 View
Does environmental protection promote economic development and relationship between environmental protection and economic development?
21 April 2024 1,964 3 View
Hi all We imported a tube of hybridoma cell line, but the courier company accidentally forgot to top up dry ice, while my cells were kept at -10 degree for approx 2 days. I tried to put the cells...
18 April 2024 3,150 2 View
I want to copy a target gene from cDNA into a plasmid. The primers were designed according to the CDS sequence from NCBI. But when I performed PCR reactions I could not get any target bands. So...
16 April 2024 2,136 4 View
Hello. I'm writing with courage to seek advice from professors and researchers. Currently, I am researching the impact of introducing a B plasmid into E. coli carrying an A plasmid, to study the...
14 April 2024 8,218 1 View
Can I use RIPA buffer to lysis insect cells SF9?
11 April 2024 6,272 2 View