The AB1 sequence file sent by Macrogen company was reverse sequence only. I can use it instead of forward sequence to deposit in the gene bank and convert it to amino acids and perform analyses on it, such as alignment and phylogenetic tree construction.
EMBOSS https://emboss.sourceforge.net is an open source software collection that contains a wide variety of sequence utilities. Its utilities are also available through the web: see https://emboss.sourceforge.net/servers/ for a list of servers.
You want to reverse complement https://www.bioinformatics.nl/cgi-bin/emboss/revseq and translate https://www.ebi.ac.uk/jdispatcher/st/emboss_transeq your sequence
(Of course, you'll find both utilities on the same server, but I just gave you the links to the first hits I got when googling for "emboss complement" and "emboss complement"
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