Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
We want to keep AML cell lines together with T cells in serum free medium for about 4-7 days. Currently we culture them in RPMI 1640 with 10% FCS, so something resembling that would be good I...
22 May 2024 1,763 0 View
I am a master student at Erasmus university and writing my thesis about branding in the banking sector. I am desperately looking for participants for my interviews, a lot of companies are too busy...
02 April 2024 3,264 1 View
Hi everyone, We are trying to count the number of cells within a ROI in the Dentate Gyrus from fluorescently labeled mouse brain tissue (sectioned at 40 microns). We have samples with cells that...
20 December 2023 7,869 0 View
I still don't figure it out yet how exactly a virus particle is formed by adding three plasmids (transfer plasmid with transgene, packaging plasmid and envelope plasmid) in a cell by...
27 November 2023 5,725 4 View
My lab is experiencing a new issue with several mouse brain tissue sections (for confirming placement, no immunohistochemistry). Each tissue section has white "sparkles" that dissipate over time...
12 November 2023 1,757 0 View
The primary objective of this inquiry is to establish the existence of a validated Medical Student Self-Efficacy Scale in the French language. This question is motivated by the significance of...
31 October 2023 5,676 0 View
I am having trouble getting consistente results with MAIPS4510 ELISPOT plates (Merck, Millipore). We have used them before with no trouble and using the same protocol. Or positive controls (PHA)...
26 October 2023 7,248 0 View
Dear all, I'm looking for an alternative for a CO2 incubator with O2 regulation as these are quite expensive and I actually just need 5% O2, 5% CO2, 37°C over 3 days every 2 weeks (should fit...
10 April 2023 5,395 2 View
Hi, I'm working on a research proposal for a pilot study and struggling to land on the optimal sample size. The quant measures (physiological measures and surveys) are recorded while the...
27 March 2023 9,408 6 View
I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.
11 August 2024 9,101 4 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...
10 August 2024 7,180 0 View
I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...
10 August 2024 7,429 2 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View