real time RT- PCR can not reach the same plateau with lower concentration template, comparing to the higher concentration template. Does MMLV has the inhibition effect on this?
Hello fellow researchers, I am working on optimizing resource allocation in multi-cloud environments through dynamic, cost-aware algorithms. My approach involves developing real-time adaptive...
25 June 2024 6,006 2 View
Hello! It is my first time on this platform and I have been wanting to know the policies and process on how I can publish my research papers and also have them peer-reviewed. Thank you very much.
07 May 2024 422 2 View
I have this error message on my Aspen plus when simulating the reactor (REqul) simulation. "WARNING WHILE CHECKING INPUT SPECIFICATIONS BLOCK NAME: B1 MODEL NAME: REQUIL PHYSICAL...
06 December 2023 5,074 7 View
Hello I'm using PC3 and DU145 cells for researching metastasis. Before the question, please understand my poor English. PC3 passage number is about 32 and I can't get more low passage number cell...
09 November 2023 5,208 2 View
Two days ago, I carried out the Agarose gel electrophoresis. And also, I performed the PCR prior to the electrophoresis (bt_3294 [KCTC 5015]). (The process that I did is on the picture.) But I got...
24 September 2023 1,966 9 View
I learned DNS assay to find out the quantity of maltose in the sample. I realized the importance about the boiling water in this assay. But I got a several question after that. I don't know the...
10 September 2023 1,505 2 View
Hello everyone. I've been using papain vials from Worthington to dissociate mouse dorsal root ganglia (DRG) neurons. (I also use L-cysteine, collagenase II, and Dispase in the same...
29 March 2023 5,160 0 View
I would like to ask for your advice on the chemical change of iron(iii) oxide during neutralization in a strong acid environment. As you know, iron(iii) oxide is red powder using as a dye. Also,...
27 February 2023 6,279 2 View
I want to add sodium bicarbonate in RPMi. I have sodium bicarbonate concentrate and it says 0.1M eluent concentrate for IC. Can I use it at human cell culture? How can I add sodium bicarbonate...
05 October 2022 3,033 3 View
I'm genotyping a line of mice and am trying to create a PCR thermocycler protocol that uses 4 primers, 2 for the WT gene and 2 for the mutant transgene. I've run this a couple of times but can't...
07 March 2022 1,030 3 View
Census data seems only to reach the year 2011, but there might be estimates somewhere that I cannot find? I am looking not only for the largest cities but also towns etc. Thanks!
10 August 2024 857 4 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...
05 August 2024 10,079 0 View
As described in the Multifractal detrended fluctuation analysis (MFDFA) algorithm, it at first calculates the profile of the time series, and then other steps are operated on the profile....
05 August 2024 9,366 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I need the python code to forecast what crop production will be in the next decade considering climate and crop production variables as seen in the attached.csv file.
05 August 2024 2,977 3 View
I want to know how do you make real time pcr solation ? is there any machine to make it? thanks for answering
05 August 2024 1,660 0 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View