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I’m currently reprogramming my fibroblast cells using Simplicon RNA OKSG-cMyc TagRFP Kit and after 13 days, these colony/aggregates started to show up in all of my wells. I have 6 wells, 2 wells...
08 July 2024 9,007 2 View
Hello, I've been doing free floating IHC using a VAChT antibody with a 1:1k dilution (139 103 - Synaptic Systems). However, there has been a struggle to keep an even stain with my samples of...
07 July 2024 4,501 2 View
When I simulate the solar cell or self-power photodetector in SCAPS-1D, I optimize all parameters, Once I check the dark current at 0 voltage, the current is high in a range of (e-16-e-25), as...
07 July 2024 1,761 3 View
How can one interpret and understand the Quran as a primary source for the life and teachings of the Prophet Muhammad, without relying solely on secondary sources or traditional commentaries, by...
06 July 2024 5,696 9 View
please suggest if there are any cracked versions of these software available.
05 July 2024 8,690 2 View
Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...
04 July 2024 3,871 5 View
I'm having a bubbling error while splicing 100/350 um optical fiber (core/cladding) on the Fujikura FSM100P+. I have tried some ways such as changing Prefuse power and Prefuse time but to no...
04 July 2024 7,443 6 View
I have already cloned ALP gene of L rhamnosus into pET22b+ vector. Cloning is already confirmed by restriction digestion and sequencing. Cloning is achieved in BL21(DE3), Codon+, Rosetta and Lemo...
03 July 2024 5,316 3 View
I have a Karl-Fischer reagent (for volumetric titration, which is a one-component reagent) with a titer of 5mg/ml. All references unanimously say the Karl-Fischer solution loses its capability...
03 July 2024 5,012 3 View
I wanted to derive pleasure and enjoyment from doing mentally challenging activities. How do I get enjoyment from doing something which is mentally difficult to do?
02 July 2024 3,940 3 View
Within our research lab we have access to PFOA in powder form and PFOS in 100microgram/L methanol solution. We want to make a PFAS contaminated water to test a PFAS treatment technology. Does...
02 July 2024 8,407 3 View
Hello everyone, I am working with mammary fat tumors from mice. My goal is to characterize tumor-infiltrating immune cells between different tx groups. I did immunofluorescence staining with...
02 July 2024 8,949 1 View
I was trying to model land cover prediction using CA-Markov in Terrset 2020, and one of the input is suitability maps that incorporated data such as elevation and slope, is anyone know how to...
01 July 2024 490 2 View
I digest my plasmid with KpnI-NotI. I cut the expected 4kb band from the gel, purified it and then I did ligation [1:3 and 1:5 vector:insert ratio] and transformation. I found 18 transformed...
01 July 2024 3,952 0 View
Is it necessary to add stop solution in Elisa plate?
01 July 2024 8,217 2 View
I know the work function and band gap of each monolayer and heterostructure. I calculated mulliken electronegativity(X) of Sc2CO2 and Bluep. From that i found Ev = X- 4.5+Eg Ec = Ev-Eg, where Eg...
29 June 2024 9,342 0 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What are the main pathophysiological changes that occur during a myocardial infarction?
16 June 2024 768 7 View
Comprehensive Overview of Myocardial Infarction: Etiology, E... What is the role of the pharmacist in the management of ischemic heart disease?
16 June 2024 4,863 1 View
low carbon emission
12 June 2024 4,907 3 View
My research project requires use of docking programs. So far I have become familiar with "Autodock Vina", as my colleagues also use it and there are numerous tutorial videos about the software....
07 June 2024 5,089 1 View
I'm currently working with gelatin sponges which have a tendency to float, however, ideally I would like them to remain at the bottom of the well plate as intend to vulture cells on them. Is there...
04 June 2024 7,712 2 View
Hello, I would like to know how to calculate the mass of an elliptical galaxy. If someone could explain the commonly used methods and formulas, it would be greatly appreciated. Additionally, could...
03 June 2024 3,227 10 View
Hi, everyone. I have some issues with THP-1 cell culture. I have little experience in cell culture and I hope some of you could share some experiences in culturing this type of cell. I'm...
31 May 2024 7,743 1 View
Metacognition is the skill of critically evaluating one's thoughts, beliefs, and knowledge, while actively seeking new insights and understanding. It involves identifying, analyzing, and...
30 May 2024 946 2 View