Transfection parameters for MEG-01 cells for CRISPR using the Neon system (Thermofisher)?

22 August 2024 0 8K Report

Hi, I am wanting to transfect MEG-01 cells for CRISPR using the Neon (10ul) system. Does anyone have any experience with what the best conditions are? eg. voltage, pulse width and number of pulses. I am also using K562s so will just try the published conditions for these on the MEG-01 line as a starting point. But if there is any infomation, it would be greatly appreciated. Thanks.

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