Does pcDNA3.1 vector not work for HEK293T cells?
https://www.researchgate.net/post/Why-there-is-low-protein-expression-efficiency-in-HEK-293F-cells
https://www.researchgate.net/post/How-can-I-make-a-stable-HEK293-cell-line-using-pcDNA31
Those discuss topics that may be helpful. Good luck.
Dear Shin Murakami,
Thank you very much for sharing the important links.
Best wishes
Puspita
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