Hi everybody,
I purified a recombinant Pathogenesis Related protein belonging to class 10 (PR-10) and I want to test if the enzyme has RNase activity.
I read that is possible to test that activity on Agarose gel under particular condition after the incubation of my protein with RNA total exctract; I want to set another protocol using a fluorimetric assay.
I found RNaseAlert™ Lab Test Kit v2 but data sheet says "The sequence of the RNaseAlert™ substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase™."
In your opinion, can I use this kit to test my protein activity or would I just throw my money?
Thanks for your answers!
Viliano Vivenzio
(https://www.thermofisher.com/order/catalog/product/4479768)
I think that kit would work just fine for measuring the RNase activity of your protein. The major advantage of using that kit rather than electrophoresis is the ability to do real-time measurements of the RNase activity.
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