Does anyone know how to prepare NAC solution for yeast cells saccharomyces cerevisiae?

19 January 2024 1 3K Report

What solvent should I use for the yeast cells? Precautions to take when preparing the solution (pH...)? concentration range for non-toxic efficacy?

Ana Luiza Franco Vilar de Queiroz

I prepare in deionizated water at the concentration of 600mM, because the solubility is near it (I think it is 660mM) and then I filter the solution to sterilize, because I think it degrades in autoclave. I use the final concentration of 20mM and it is not toxic and improves the growth in oxidative stress conditions, but other people already saw this effect at less concentrations, like 10mM.

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