I currently need to purify DNA after plasmid linearization. I am using the TaKaRa DNA purification kit, but the concentration of the diluent obtained from each DNA purification is very low. Do you have any other recommended kits?
You can elute in a smaller volume of buffer.
You'll also get better yield with a slight variation on the protocol.
Heat the buffer to 60*C, add buffer to the spin columns, let them sit 1 minute, then spin to elute.
Also, make sure you are not over-loading the column. There is a limit on how much starting culture you can use. Check the user manual that came with the kit to know the exact details.
Good luck!
Most dna purification kits use the same technology and all give low purified dna yields. You can often increase the yield by heating the elution buffer to 70c and put it on the membrane for twice as long as the kit recommends. Then do the same elution again with new hot elution buffer and this should increase your yield
Try eluting in smaller volumes and warm the buffer to 60-70C.
Good luck!
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