Hi scientific community,

I have defrosted (from -20ºC) one bottle of cRPMI (complete RPMI media) then I have prepared all the aliquots (10 mL) under sterile conditions and all of them have been preserved at -20ºC.

I have defrosted two aliquot one week after aliquoting and both are very pink (colour appearance). It could be by using an aliquots preservation at -20ºC after defrost?

All the opinions/experiences will be wellcome.

Thanks in advance.