UV-Visible Spectroscopy

UV-Visible Spectroscopy refers to absorption spectroscopy in the ultraviolet-visible spectral region of the light spectrum. | Contact experts in UV-Visible Spectroscopy to get answers

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Questions related to UV-Visible Spectroscopy

Can total phosphate (TP) in soil be determined with UV-Vis Spectrophotometer?

Dear All, I would like to know if total phosphate (TP) and total nitrogen (TN) in soil can be determined with UV-Vis spectrophotometer or not. If somebody knows the method, I would like to get...

04 January 2021 7,801 6 View

How can we calculate the stoichiometric ratio of MoOx from UV-Vis diffuse reflectance/absorption spectra?

I have read one research article by M Dieterle et al. In this article they have discussed about finding stoichiometry of MoO3-x using DR UV-Vis spectra. I am not getting the required equation they...

19 December 2020 9,948 2 View

Why have a specified percentage absorbance to be reached for flow test on UV Vis?

When performing a flow test the objective is to find the total amount of blank to be replaced by sample with pump. Why would a specified percentage of 100% i.e. 99.5% of that absorbance be used?

18 December 2020 7,958 3 View

Determination of menthol using uv vis spectrophotometer?

Is there any procedure for the quantitative analysis of standard menthol (drug) in methanol using a UV vis spectrophotometer? Thanks in advance.

01 December 2020 5,476 1 View

How to explain "Localized Surface Plasmon Resonance" phenomenon of silver nanoparticles in transmission mode of UV/Vis spectroscopy?

The UV/Vis spectrum of my AgNP samples show a global curve at around 450-500 nm, which should be attributed to LSPR of silver, but how could I explain this phenomenon without reflective UV/Vis...

25 November 2020 848 2 View

What kind of compound I can use to detect k3EDTA in aqueous solution?

Any recommendation for detection of K3EDTA in aqueous solution? It would be nice to quantify it by photometric (UV-Vis) regression analysis. The compound should have no effect on the pH of the...

19 November 2020 9,029 3 View

Why are plasmonic photocatalysts always Au and Ag?

In all the literature I've read, I always find Au and Ag as metals for surface plasmon resonance photocatalysis. Why are other metals never used? One option I've considered is that other metals...

18 November 2020 4,797 3 View

UV Vis of ferrites?

how can calculate band gap using Uv-Vis . ferrites not solvable in water . In which solution disperse the ferrites for Uv vis.

11 November 2020 3,497 3 View

Can we measure mass deposited on a QCM-D sensor with a coating technique in air?

In short: 1 We measure the frequencies of the overtones of an empty gold sensor (from n1 to n13). 2 Then, we add some chemical of compound eg. oil by spin coating. 3 After this, we measure the...

10 November 2020 3,226 0 View

What is the catalytic potential of Ag nanoprisms?

i am doing a project in the catalysis of silver nanoparticle...

09 November 2020 4,625 6 View

Why does silver nitrate turn brown upon addition of leaf extract but no increase in absorption at 400 - 500 nm?

I am currently biosynthesizing silver nanoparticles using ethanolic leaf extract. Upon addition of the leaf extract to 1mM silver nitrate, the solution turns brown in color after exposure to...

09 November 2020 1,146 6 View

Why the same amount of PBS are added during drug release measurements using a UV-vis spectrometer??

I want to use UV-vis spectrometer to measure the concentration of drugs released from nanoparticles in each time point. I wonder why they added equal volume of fresh PBS as they extract for each...

28 October 2020 3,698 4 View

Does the Kubelka-Munk function F(R) represent actual absorbance of UV-Vis reflectance spectra of cyanobacteria?

Hi there, in an experiment I used a Cary-60 UV-Vis Spectrophotometer (Agilent Technologies) to collect UV-Vis reflectance spectra of cyanobacteria (~5µm cell diameter) on cellulose nitrate...

21 October 2020 5,155 1 View

I measured membrane chitosan who modified tannic-acid and absorb Cu(II) in UV-Vis spectrometer with wavelength 400-800nm, why the peak not smooth?

I synthesized a transparant membrane from chitosan which was modified with tannic acid ligands and cross-linked with tripolyphosphate to detect Cu (II) metal in solution. When I dip this membrane...

13 October 2020 7,712 7 View

Any clue on how to calculate X-ray absorption spectrum using Gaussian software?

For the best of my knowledge, the absorption (excitation) energies calculations (using Gaussian) are performed on outer shell electrons therefore we obtain "simulated UV-Vis spectrum". But I need...

02 October 2020 679 3 View

How to calculate the nanoparticle size using only UV-Vis data?

The graph obtained from UV-Vis analysis of silver nanoparticles is more or less similar for all three pH set viz., 8, 9, and 10. So I wanted to know whether there is any way to select the best pH...

01 October 2020 8,111 3 View

How can we confirm biomolecule encapsualtion into liposome from UV-Vis or PL spectra?

I have been working with phospholipid vesicles and encapsulation of bio-molecules or drugs. But, i am unable to ensure whether it is taking place or not. what are the methods to do so including...

21 August 2020 7,192 7 View

How do we distinguish between d-d and MLCT transistions?

For complexes, which have red/orange colour, the absorption for dd occurs nears 420-450 nm. However how do we know it is not MLCT but dd.

16 July 2020 2,206 2 View

Role of intensity in UV-Vis Spectroscopy?

I have two suspensions of Gold Nanorods in Deionized Water synthesized by the same experimental route. Each of the GNR solutions shows the same peak but with different intensities. Is this related...

15 July 2020 9,810 0 View

What can be the reason for increase in spectrophotometer absorbance for the same solution with the same pollutant concentration?

I am using a UNICO SQ-2800 single beam spectrophotometer and a quartz cuvette. before, I had measured the absorbance of a 10 ppm methylene blue solution (DI water solution) at its peak wavelength...

10 July 2020 5,106 6 View

Bandgap Calculation from UV-Vis spectra data?

Bandgap Calculation from UV-Vis spectra data I have UV-Vis spectra of perovskite thin-film on glass substrate and need to calculate the bandgap. Can someone give me info on how to go about this?

01 July 2020 9,683 8 View

UV-Vis Spectroscopy of Carbon Nano Tube ?

Dear all, I just started the UV-Vis Spectroscopy of Carbon nanotube (CNT). I just got confused to interpret the results. Would you please help me with these questions: 1- Why I cannot find the...

01 July 2020 4,829 7 View

How to calculate absorption coefficient from the UV-Vis absorption data ?

29 June 2020 8,495 14 View

How do different solvents impact the absorption spectrum of a solved molecule?

I have measured UV/Vis-absorbance spectra of an organic molecule (IT-4F, n-type acceptor material for organic photovoltaics) in different solvents (chlorobenzene, chloroform, toluene and...

24 June 2020 3,750 6 View

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