UV-Visible Spectroscopy

UV-Visible Spectroscopy refers to absorption spectroscopy in the ultraviolet-visible spectral region of the light spectrum. | Contact experts in UV-Visible Spectroscopy to get answers

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Questions related to UV-Visible Spectroscopy

How to incorporate the dilution factor in the final calculation after getting the calibration curve from UV-Vis?

Let's say I am testing for fluoride in a water sample. I diluted the sample 10 times by making 10ml of sample into 100ml. I take absorbance of this diluted sample using UV-Vis. So, my doubt is,...

04 September 2021 8,917 5 View

Does a material stored over a long period of time can suffer or experience Quantum confinement effect?

I have stored a material over some time. When I checked its band gap via UV-Vis, it came out to be around 3.8-4 eV rather than 1.2-1.9 eV. Are there any chances that if a material is stored over...

04 September 2021 5,614 4 View

How to make bacterial growth OD curve ?

I want to evaluate the antibacterial activity of metal nanoparticles at different time intervals. I am performing this experiment in a flask and after the specified time take out the sample for...

13 August 2021 4,342 5 View

Would you use field capacity to evaluate soil workability?

I seek a relationship between soil Field Capacity (FC) and Lower Plastic Limit (LPL) or any other physical characteristics of a soil which can help evaluate soil workability. Would you use Field...

01 August 2021 4,566 3 View

Judd–Ofelt analysis ?

I have absorbance vs wavelength (350-850nm) from UV-Vis spectroscopy measurements and also PL Excitation and Emission spectra for RE3+ doped phosphors. As per the literature, I am calculating the...

19 July 2021 1,826 5 View

Gold nanoparticles losing binding capacity over time?

I have made these 40 nm GNPs which I purified via dialysis until no traces of contaminants were detected by UV-vis. The GNPs do not aggregate over time (lambda max does not change, neither does...

06 July 2021 8,315 6 View

Is it possible to have UV-Vis spectra calculations including media viscosity?

I would like to perform a computational study of how solvatochromism is affected by viscosity of the medium. I usually use Gaussian as software for molecular modelling, and my question is whether...

04 July 2021 9,430 3 View

What is the laboratory protocol analysing biochar dissolved organic carbon using UV spectroscopy?

The protocol, using three extraction solutions (water, acid and alkaline base), using freezing drying and UV spectroscopy

21 June 2021 5,681 3 View

What types of effects occur in its optical properties on doping metal oxide in organic semiconductors?

need experts answer what types of effects occur on its optical properties on doping metal oxide in organic semiconductors? why and how blue shift occurs in the UV-vis spectrum on doping in...

18 June 2021 8,442 3 View

Hello , I am currently looking for a way for a analysis at UV_Vis. data.Can anyone suggest any software available?

Can anyone suggest any software available?

12 May 2021 9,776 4 View

Parts for Lambda 365 - stepper motors?

Hi. The Lambda 365 in our lab has some issues - namely, depending on the occasion either with slot calibration or with the D2 wavelenght calibration. The elements that drive the grating and the...

07 May 2021 4,620 3 View

On hydrolysis, changes in UV-Vis spectrum is occurring but not in NMR and mass. Why is it happening?

For my organometallic complex of the type [RuCl2(Z)], where Z is a neutrally coordinated ligand, I am observing distinct changes in UV Visible spectrum upon hydrolysis (complex is added to water)....

06 May 2021 6,572 8 View

How to prepare solid sample for UV test? Why UV-Vis absorption show negative value?

I dispersed my sample in IPA and dropped onto Si wafer. I wonder if this is the right way to prepare sample? When I check UV-Vis spectrometry, I used reflectance mode and convert it to absorption,...

04 May 2021 4,928 9 View

Has anybody tried using Nanodrop for scaling down absorbance-based assays (i.e. biofilm quantification)?

I don't have a microplate reader in my lab now and using a cuvette-type UV-Vis spectro is kinda "wasteful" in terms of how much of the treatment solutions I'll be using. So I thought of using a...

03 May 2021 9,591 3 View

What are some possible substrates that can be used to test laccase activity?

I want to know some cheap, easily available compounds that can be used as substrates to detect laccase activity instead of ABTS using UV-Vis spectrometer.

28 April 2021 266 3 View

How many points and of what concentrations should I take for calibration curve for UV-vis spectrophotmeter to determine the NO3- ions conc. in water?

Hi, I expect a concentration of 5 mg/L of Nitrate ions in a deionized water sample, containing only one solute i.e. NaNO3. How many points should I take and what respective concentrations to...

12 April 2021 1,783 5 View

I don't know why the textured pdms total transmittance obtained by UV vis spectrometer exceeds 100%, can you help?

Instrument: UV-vis-NIR spectrophotometer (Cary 5000, Agilent) (DRA accessory used) Sample: Textrued PDMS thin film on glass Reference: Air Air has 100% transmittance in all wavelength ranges....

17 March 2021 7,647 5 View

How to get to know whether Fe3O4 or gama Fe2O3 is form by reacting Fe+2 and Fe+3?

how to characterized whether Fe3O4 or Fe2O3 is formed by reacting FeCl3.6H2O and FeCl2.4H2O(2:1) in the basic medium under a nitrogen atmosphere? will it be characterized by using IR or UV...

12 March 2021 1,910 7 View

What concentration of sample is required to measure the absorption spectrum on a UV/Vis Spectrophotometer?

I am trying to measure the absorption spectrum of a photo-caged fluorescent probe on an Agilent Cary 100 UV-Vis. I tried a 10mM solution in DMSO after a DMSO blank and the spectra had a broad flat...

11 March 2021 10,040 5 View

UV-Vis Spectroscopy for metal analysis?

Is it possible to determine the concentration of metal in a metal complex using UV-Vis spectroscopy???

08 March 2021 4,089 3 View

Does the crystal structure of MOF differ depending on the presence or absence of substituents?

Currently, I am conducting physical property research on the premise that the crystal structure of multivariable MOF (MTV-MOF) is the same. The paper states that MOF does not change its crystal...

06 March 2021 2,287 1 View

What dilution do I use for my GNP solution UV-Vis and DLS characterization?

I have prepared gold nanoparticles using the citrate reduction method in the lab. I need to characterize them using UV-Vis and DLS, by how much should I dilute my sample for using it in the...

27 January 2021 795 5 View

Best glass slides for metal oxide UV-vis and PL measurements?

Hi there, I am currently using Alfa Aesar fused quartz slides for my UV-vis and PL measurements of TiO2, but it seems that their slides emit in the UV around the same wavelength region where I...

20 January 2021 723 3 View

How good repeatability can be ensured for Tetracycline (TC) degradation study in UV-visible spectrophotometer?

Hai all, I am using 50 ppm Tetracycline hydrochloride for the degradation study ( Fenton process). After each interval sample taken, filtering through 0.22 micrometer syringe filter, then...

08 January 2021 4,140 2 View

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