How to model a fully Binary dataset?

I am having a difficult time modeling a binary dataset that my team has gathered. It is a dataset of different prescriptions per each patient and an outcome of a certain event. The goal is to...

02 February 2020 9,388 4 View

How may I clone a 5415 bp gene into pglo plasmid (5371 bp) replacing it's GFP gene (~700 bp)?

I need to clone a 5415 bp gene into pglo plasmid replacing it's GFP gene which is around 700bp long. Now PCR amplification of this 5415 bp gene using PhusionR HiFi Taq doesn't show any band, only...

05 October 2019 4,938 7 View

Can anyone suggest me regarding my DAPI staining cell? I would like to know identification of the morphology of these cells?

I stained the cells with DAPI after treated with chemicals and then checked with fluorescence microscrope. I would like to know what is the morphology of these cells.

09 August 2019 9,328 5 View

Are there any published studies using the 20-item Values Inventory since it was first described in 2016?

IF SO, CAN YOU CITE THEM?

07 April 2019 4,062 1 View

Only one peak in heavy fraction is acceptable for qPCR result for DNA-SIP?

Hello, I am doing DNA-SIP for 13C labeling soil samples lately and I checked the SCI papers which have done DNA-SIP work. Generally, after ultracentrifugation, we do density gradient fractionation...

17 March 2019 3,387 2 View

Reliability and validity of an invented survey?

I want to implement a quality improvement protocol, which includes education, on the provision of oral care in an acute care facility. I would like to conduct a pre- and post- survey to the...

16 January 2019 9,250 2 View

Diffusion distance of rice root exudates?

I would like to know the diffusion distance of rice root exudates. How far can rice root exudates affect the microorganisms in bulk soil? I do not mean for soil depth, just horizontal distance. I...

24 November 2018 2,867 5 View

Is it possible to use SSR to investigate the bacterial diversity in soils?

06 August 2017 2,087 4 View

Does anybody know how to separate with highly polar compound and salts.

Recently, i isolate highly polar compound from n-butanol fraction. It's retention time is 5 min with buffer (0.1M H3PO4: 0.1M KH2PO4) 95 and MeOH 5. Because of i used buffer, compound will be...

29 June 2017 925 2 View

How to use software Geneious to find genomic features?

How to use software Geneious to find genomic features such as genome size, GC content, tRNA genes, CDS etc?

10 June 2017 1,062 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Why do a weight decrease for thermal degradation in nitrogen gas condition?

Hi all The question is related to TGA(Thermogravimetric analysis). Thermal degradation easily understood but why do samples weight decrease? altough just binding is broked, the weight of...

26 February 2021 1,100 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View