UV-Visible Spectroscopy

UV-Visible Spectroscopy refers to absorption spectroscopy in the ultraviolet-visible spectral region of the light spectrum. | Contact experts in UV-Visible Spectroscopy to get answers

1,453 views 868 posts

Questions related to UV-Visible Spectroscopy

Does anyone know where I could find molar extinction (absorption) coefficients for iodine vapor (I2) for UV-Vis spectroscopy?

Can't seem to find any numbers anywhere.

13 May 2019 1,678 3 View

Why is my UV-Vis results for bixin so different?

I made a 1:10 ratio solution of Annatto seeds and Methanol. When i used the UV-Vis to measure its Absorbance, i found out that the results were so different from those of related literature. My...

07 May 2019 5,499 5 View

Dear researchers, I get my UV-Vis DRS results of my materials. So i get F(R), is it allow to plot tauc question directly using F(R) withou absorbance?

i have F(R). can i plot tauc equation with F(R) without convert it into absorbance? What represent F(R)? is it also absorbance according to K-M equation?

06 May 2019 1,979 1 View

Is this the correct method to determine the nanoscale particles size using UV-Vis Spectroscopy?

If yes, how can you find the particle size distribution in it?

03 May 2019 6,111 3 View

Can any one explain the reason behind negative OD values at certain regions of the UV-Vis spectra?

I got positive OD values for pure sample. But I got negative OD values at certain regions of the spectra upon addition of nanoparticles successively in both the reference and sample cuvettes. what...

15 April 2019 8,191 11 View

Which solvents are useful to dissolve liposomes (lipid vesicles) with protein without precipitating the protein?

Hi. I'm encapsulating a protein inside a liposome (lipid vesicle). I want to quantify encapsulated protein by UV since it absorbs and the lipids don't. But I need to dissolve both the liposomes...

05 April 2019 3,836 10 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

20 March 2019 8,266 3 View

Why is value of my blank higher than value of my samples in UV vis spectroscopy?

I am trying to plot a graph with R^2 > 0.8 with increasing values of Cadmium chelate from 0,2,4,6,8,10 mg/l . According to a paper I have read Cadmium-Alizarin Chelate is used in this method so...

17 February 2019 2,535 3 View

Can i reused sample measured by UV spectroscopy ?

my sample is small, can i reused the sample that already measured and get the uv on it???? for other test used can i??

11 February 2019 1,057 4 View

What are the characteristic absorption ranges of nickel and Nickel oxide nanoparticles?

How to differentiate between the absorption peak of Nickel and Nickel nanoparticles? Which peak will be appeared first (Nickel or Nickel nanoparticles in the absorption spectrum of UV-Vis...

08 February 2019 2,439 3 View

How can I view the absorption peaks of highly diluted suspension liquids?

I had synthesized MoS2 nanosheets by the help of liquid phase exfoliation using probe sonication. The solvent used was a 50/50(vol%) of IPA/DI water mixture. Pre-processing of the sample was...

31 January 2019 5,541 5 View

How can I open Genesys 10S UV-Vis *.ss spectra files?

I have been scanning UV-Vis spectra on a Genesys 10S & have saved the *.ss data files onto a USB. However, my computer is unable to open them. The VisionLite 5 software from Thermo Scientific...

31 January 2019 8,011 3 View

Anyone please suggest a simple method to count cell suspension through UV-VIS Spectrophotometer...?

28 January 2019 1,320 4 View

How to perform Encapsulation efficiency of liposomal drugs other than centrifugation method?

I have been working on EE studies for my liposomal drug. Size of the particle is around 70-100nm and I measure my drug concentration using UV-Vis method. I have tried centrifuging at ~17,000g for...

27 January 2019 7,182 14 View

I have Cadmium +2 ions in a solution, What possible compounds can I add to this solution to make UV spectroscopy so as to find how much Cd +2 absorbs?

According to my teacher and research papers I have come to understand that UV spectroscopy requires Cadmium to be in a Chelating compound or a larger compound . What possible compounds can I add...

25 January 2019 4,833 2 View

What factors did the intensity of UV absorption depends on?

Hi I have a halogen interacted Heme for different charge of Fe. I find the intensity of the soret peak is no way uniform and it varies for every complexes. So i have few questions based on...

25 January 2019 8,392 3 View

Excitonic peaks of MoS2 in C/MoS2 composite?

Dear researchers Can anyone tell me the effects of carbon nanoparticles on the excitonic peaks of the MoS2 nanosheets in C/MoS2 composite?

15 January 2019 7,853 1 View

How do you measure polymers erosion through UV absorbance?

I'd like to quantify the erosion products of the polyethylene glycol (PEG) end-modified with ureidopyrimidinone (UPy) moieties. I've read in a previous study that this can be done by measuring...

09 January 2019 2,630 1 View

Why is there anincrease in FWHM uv peak of Fe(pyridine terpyridine)2 complex on immoblizing it on glass substrate ?

complex had fwhm of 40 nm in acetonitrile increase to 60nm immobalising covelently on glass substrate

07 January 2019 381 1 View

How do I know if my data obey Beer's law or not?

Hi all, I would like to know if the data, for a given drug, obtained from the UV/VIS Spectrometer obey Beer's law or not. Is there a general rule? Also, why an Absorbance Reading above one is...

30 December 2018 4,547 6 View

How to prepare deoxyhemoglobin by nitrogen bubbling?

I prepared deoxyhemoglobin by mixing a few drops of Stokes' reagent into a certain concentration of oxyhemoglobin solution. The deoxyHb was confirmed by UV-vis spectroscopy. I also tried to use...

21 December 2018 6,364 7 View

What are the factors deciding the bandgap of quantum dots?

Quantum confinement effect plays an important role on band-gap of quantum dots. but in some conditions, even though the size of the particle is reduced due to doping the optical band gap not...

20 December 2018 9,956 19 View

Why am I getting additional absorption peak (bump and valley kind of structure) in GaAs sample implanted with 50 keV silicon ion ?

We have implanted 50 keV silicon ion with fluence of 5e15 ion cm-2 in GaAs sample. The UV-Vis graph (alpha2 Vs. photon energy) showed additional absorption peak (between 1.35 eV to 1.37 eV ). What...

13 December 2018 7,839 3 View

How to check valence state of Cr using UV vis spectroscopy?

I want to ask about how to get the accurate valence statr of Cr in solution form? As from XPS I found it is +3 but somebody ask that Cr+3 can not be attached on material in acidic solution. So ask...

11 December 2018 2,643 3 View

Badges
Science method