UV-Visible Spectroscopy

UV-Visible Spectroscopy refers to absorption spectroscopy in the ultraviolet-visible spectral region of the light spectrum. | Contact experts in UV-Visible Spectroscopy to get answers

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Questions related to UV-Visible Spectroscopy

Simulating fluorescence emisssion spectrum when Excited state is sufficiently different from Ground State geometry?

Dear All, I am trying to obtain the fluorescence emission spectrum of a molecule using Gaussian. I have tried the default Franck Condon methods and the vertical hessian methods available in...

27 November 2018 8,035 0 View

How to measure Arsenic in water using UV VIS spectrophotometer?

I used Heteropoly Blue method but it do not detect lower concentrations of arsenic.

20 November 2018 6,248 5 View

DMMB assay for Chondroitin Sulfate - standard curve not linear?

Hello, I am trying to measure chondroitin sulfate concentrations in supernatant of cell cultures (minimal media = Phosphate buffer, MgSO4, NH4CL, and Chondroitin sulfate, pH 7.0). I am using the...

19 November 2018 890 2 View

How can i calculate band gap from UV-Vis absorption spectroscopy? In my case sample absorbance spectrum is got by dissolving powder in liquid?

16 November 2018 9,653 3 View

Which solvent is suitable for dissolving mono phosphates like sodium calcium orthophosphates?

I wish to record UV-Vis spectra of my sample in solution form instead of DRS .So I am willing to dissolve my powder sample in a suitable solvent. Please suggest me, which are the suitable solvent...

09 November 2018 1,261 2 View

What is the relationship between wavelength absorption and temperature of CdSe QD?

I synthesized CdSe QD at 40-90 degree celsius and their UV-vis absorption wavelength was increased with increasing temperature. I wanna know what is the relationship between wavelength absorption...

07 November 2018 2,270 5 View

How to detect unbinding of a protein from a gold nanoparticle through UV-Vis?

From literature, it could be observed that while binding of gold nanoparticle and protein occurs the UV-VIS peak of gold shifts by a small amount suggesting binding. Although further...

16 October 2018 8,768 3 View

Why TDDFT calculation does not match with the experimental UV-Vis spectrum?

I have calculated the structure of a metal(Ag) complex for which the crystal structure data is available with us. The UV-vis spectrum of the complex shows a very strong absorption at ~250 nm and a...

09 October 2018 389 3 View

In montmorillonite colloid and polystyrene colloid mixing system, how to quantify the two separately?

Montmorillonite colloids and polystyrene colloids have similar UV absorption wavelengths. It is difficult to distinguish and quantify them by UV-Vis spectrophotometry. Is there a simple way to...

19 September 2018 5,844 4 View

What tools should preferably be used to detect nitrates in drinking water ?

AAS, GC-MS GC-ECD UV-Vis HPLC etc

11 September 2018 3,426 9 View

How to connect UV-Vis 10 Thermo to PC?

We have a UV-Vis 10 Spectroscopy of Thermo Company, but we don't know any software to connect and control by PC or laptop.

03 September 2018 3,147 4 View

i wanted to study the photocatalytic property of a compound whose band gap is around 1.7 eV.My question is what would best dye to use ?

I tried with rhodamine B with irradiation source 50 W LED but could not observe any change in the UV-Vis spectrum for the sample taken out after interval of 1 hour each. even for sample irradiated...

30 August 2018 5,789 6 View

What are the techniques to reduce the size of the particles from micrometer to nanometer range?

Hi Everyone, I have Boron phosphide in powder form and to determine its band gap using UV-Vis spectroscopy, I grinded the particles first and then dispersed them in DMSO but I didn't observe any...

22 August 2018 3,184 7 View

How to find optical energy gap of metal complex using UV-vis diffuse absorbance spectra?

I have obtained wave length vs absorbance data for powdered metal complex using UV-vis spectrometer (DRS). how to find optical band gap using kubelka-mulk function.

19 August 2018 5,171 2 View

If non covalent molecular association give rise to new UV-Vis absorption peak and new PL emi, can we term this as quantum confined supramol str?

quantum confinement in soft supramolecular structures

04 August 2018 892 7 View

Does binding of a protein to a gold nanoparticle change the color of gold nanoparticles?

Binding of a protein to a gold nanoparticle(5-7 nm) increases the size of the complex as validated by a red shift in the uv-vis spectra. Does the overall size increase of AuNP-Protein complex...

31 July 2018 7,868 3 View

Measuring scattering and absorption?

Hi, We have a UV-VIS spectrophotometer in our lab. The measurement it gives is basically extinction. I am interested in measuring absorption and scattering separately instead of extinction of my...

23 July 2018 4,293 3 View

What should be the y-axis title: Kubelka-Munk or absorbance if I convert Uv-Vis diffuse reflectance spectrum to absorbance by Kubelka-Munk function?

I have collected the UV-Vis diffuse reflectance spectrum of a insoluble powder sample and then converted to absorbance by Kubelka-Munk function. What should be the y-axis title: Kubelka-Munk or...

22 July 2018 5,501 8 View

Is there any library to find the molar extinction coefficients of compounds?

I need the molar extinction cefficient of 3,5-di-tert-butyl-1-oxacyclohepta-3,5-diene-2,7-dione

20 July 2018 983 4 View

Is there a quantitative color test for the presence of urea in a solution ?

I am trying to co-relate the change in concentration of urea in a solution over time using UV-Vis spectrophotometry. Unfortunately, the absorbance peak for the same lies below the detection...

20 July 2018 7,490 3 View

What is the accurate method for quantification of protein during immobilization?

There is a problem i am getting during the immobilization of protein on support . Initially 1-2mg/mL of protein concentration and 1 mg of support is being taken. After immobilization the protein...

09 July 2018 2,686 3 View

Absorbance measurement for anaerobic culture?

I am cultivating a culture of anaerobic archaea. The growing medium includes sodium resazurin as anoxic indicator. I would like to estimate the cell concentration of the culture by measuring the...

29 June 2018 4,966 3 View

Using UV-Vis, where does elemental Sulphur peak occur?

I'm doing experiment on Sulphur dissolution in ionic liquid solvent. I need to find the sulphur solubility using Uv-vis. How can i measure the presence of S in the mixture?

23 June 2018 7,751 3 View

What is the lambda max (absorbance peak) in UV region for the following samples?

I performed UV-Vis spectroscopy for following samples.What is the right method to find lambda max

20 June 2018 3,409 3 View

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