Absorption Spectroscopy

Absorption spectroscopy refers to spectroscopic techniques that measure the absorption of radiation, as a function of frequency or wavelength, due... | Contact experts in Absorption Spectroscopy to get answers

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Questions related to Absorption Spectroscopy

Handheld Portable Raman spectroscopy systems - recommendations and experience?

Hi all, I am looking for some general feedback and information from those that have experience using handheld/portable systems? I am looking at the prospects of using an off the shelf system from...

08 February 2021 9,898 3 View

How do I analyse a Photoluminescence Spectrum ?

I have a few questions related to PL (images attached for same material by 2 methods), It would be great to get some help. 1. What is the physical meaning of highly intense PL? Does this mean...

03 February 2021 1,997 3 View

What type of spectral radiation has greater penetration on materials, UV or IR? What book or source do you recommend for information on this?

I need information concerning the penetration and reflection capacity of ultraviolet and infrared radiation wavelengths on different most common materials. Edit. Can somebody recommend a book to...

17 January 2021 8,273 6 View

Conversion of Absorbance to Concentration?

Hi, I recently calculated the absorbance of chitosan nanoparticles with encapsulated beetroot leaf extracts from UV Spectrophotometer but I need the concentration to calculate the amount of drug...

13 October 2020 9,824 3 View

Can anyone suggest a wavelegth which is most effective to measure/detect H2O using TDLAS in a shock tube experiment?

I have seen people using 1.39 µm, 2,5 µm respectively. Currently, I am working with 1,392 µm to measure H2O. Also, one can choose the appropriate wavelength from the 'spectraplot' as well. Can...

03 September 2020 3,634 3 View

Can any one explain the difference between vibration modes and assignments in infrared analyses?

When I read this short paragraph, ''- Vibration modes was in Greek letters for stretching and bending vibrations etc; Use amide I, II and III identifications. - Assignments like...

30 August 2020 3,168 5 View

How to predict accurate Fluorescence/Vertical Emissions employing TD- DFT? Excited State Tuning Approach?

How can we predict accurate Fluorescence wavelengths employing DFT? Which is the most accurate DFT methodology currently available which can predict accurate Fluorescence wavelengths (Vertical...

06 August 2020 4,519 1 View

FTIR-ATR efficiency in the analysis of bone samples?

Due to the lack of access to my university's laboratories and equipment I'm exploring the efficiency of FTIR in the characterisation of bone samples from reviewing literature as opposed to...

16 July 2020 2,190 1 View

Why the band gap of my sample decrease after increase with the rise of pressure?

Here I have a question about band gap of metalic oxide semiconductor, The UV-vis absorbtion spectrum shows that the band gap of my sample increased with pressure, but the band gap decreased...

04 January 2020 527 3 View

Does anyone know if there is a reliable method to determine oil concentration (crude oil to be precise) using a UV-Vis spectrophotometer?

happy new year fellow researchers, I'm working on treating synthetic oily wastewater made from mixing distilled water, crude oil and surfactant.

01 January 2020 6,468 3 View

What is the best optical material for the in region 6-12μm and temperature around 1000 K?

I want to develop an optical cell for high pressure and Temperature (P=10 bar, T=1000 K) in region 6-12μm. Could you please suggest me specific optical window for the given criteria? Since I would...

30 November 2019 9,514 2 View

Maximum dimensions for (Bruker) FTIR-ATR samples?

I am curious about the maximum recommended dimensions for ATR samples? I am planning to analyse several small animal bone samples and I'm hoping to have minimal preparation/fragmentation of the...

09 November 2019 1,242 1 View

What does the 3rd peak in a PL excitation spectrum mean (image attached)?

This is the case of a wide bandgap, natural silicate material (~7.7 eV). The excitation spectrum is recorded for a defect (depth from the conduction band edge is ~ 2.1 eV). Any leads will be...

05 September 2019 7,366 3 View

How to do blank correction when the absorbance values increase with decrease in the concentration?

I am working on anti-factor Xa activity assay. The absorbance values increase with decrease in the concentration, and the blank values are always higher than the rest of the concentration. How...

23 August 2019 9,112 3 View

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19 June 2019 7,552 8 View

What are the additional advantages that a Fano resonance possesses in comparison to normal dipolar resonance in Optical systems?

Fano resonance is a distinctive spectroscopic behavior in many optical systems. There're certain differences in terms of physics and behavior of Fano resonance compare to dipole resonance which is...

31 May 2019 4,444 2 View

Which part of solar spectrum(wavelength) does snow (on high altitude mountains) absorb and melt?

We know that heating happens due to mid-infrared region of solar irradiation. Ice melts due to absorption of which specific range of wavelengths of electromagnetic spectrum? Can we relate this...

18 May 2019 7,609 3 View

Which free software can use for optical emission spectroscopy (OES) analysis?

Which free software can use for optical emission spectroscopy (OES) analysis? I am looking for On-site OES analysis software having a built-in elemental library.

07 May 2019 2,552 10 View

How to quantify hydrogen peroxide using potassium iodide, ammonium molybdate, and leucocrystal violet?

I'm attempting to follow the method outlined in the following publication for determining the concentration of hydrogen peroxide in aqueous solution: Mathew, Sunitha B., Ajai K. Pillai, and...

24 April 2019 6,268 2 View

How do I measure "extinction spectra" for a graphene dispersion?

Hi everyone, I am reading an article which has measured "extinction spectra" for a dispersion of MoS2, but hasn't explained the details. I wish to measure the same spectrum for a dispersion of...

28 December 2018 1,624 4 View

"kit of pyruvate concentration" for serum samples are good or suitable for cell line suspension??

Have a nice day colleague i'm going to determine pyruvate concentration from cell line suspension (before and after treatment) for different duration times using Elabscience pyruvate assay kit...

21 October 2018 9,581 2 View

What is the area of beam?

I want to calculate the intensity of light with this equation, I=P/A where P is the power of source and A is the area of beam.

04 September 2018 7,530 3 View

Is there a method to measure the optical properties of films printed via fused deposition modeling (FDM)?

I have tried to collect (transmittance and reflectance data) with a Filmetrics RT-10 spectral reflectometer on FDM printed t-glase thin film. However, an application engineer from the company...

22 August 2018 5,397 4 View

Amplitude of interference fringes in transmission spectra of uv-cureable varnishes. Why do they appear after referencing against layer a. substrate?

Hi, I have the following setup: A polychromatic light source, a uv-cured thin layer of a transparent uv-varnish on quartz-glass (6mm). The light beam has an angle of 0 degree to the sample. The...

08 June 2018 2,623 3 View

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