Has anyone used Pro-Touch color bands to mark raptors?

We have been color banding American Kestrels using A-craft alpha-numeric color bands since ~2013 and would like to test other options. I am curious if any other researchers have experience using...

01 February 2021 6,786 3 View

Which LCIA method is most suitable for assessing the impacts of nitrate leaching in groundwater (eutrophication )?

The eutrophication of groundwater by nitrate leaching is major environmental problem in Germany, especially in areas with intensive agriculture and high N-fertilizer inputs. In addition there are...

16 January 2021 5,182 7 View

Do Radial Basis Function Networks trained through Gradient Descent benefit from Logistic Regression?

When training a Radial Basis Function Classifier for classification problems, we generally train the model by making use of the pseudo-inverse of the design matrix, minimizing the MSE error...

09 December 2020 2,190 1 View

Repeated measures ANOVA with technical replicates?

I am doing an experiment where I measure the blood pressure of a treatment and control group over the course of several weeks. At each measurement session I collect multiple recordings. From what...

06 December 2020 531 7 View

Qiagen Viral RNA extraction kit showed similar results for positive and negative control. How should I proceed?

Our team is looking for viral RNA in the environment. We took swabs off of doorknobs using cotton dipped in water and then submerging that swab in viral transport media. We also wiped 1 surface...

08 June 2020 6,532 3 View

How do I determining reaction orders?

Our lab had run some experiments to electrochemically degrade some compounds. When I plotted the data to see if the ractions were first or second order, the R-squared values were generally about...

04 May 2020 2,809 3 View

MitoSpy vs. MitoTacker?

I have a very long and rich history using MitoTracker dyes, but I had not, until recently, even heard of MitoSpy. Has anyone used this dye side-by-side with any of the MitoTracker? Is MitoSpy the...

25 November 2019 3,412 2 View

Where can I purchase syndiotactic polypropylene (sPP)?

Hello, I'm looking to purchase syndiotactic polypropylene (sPP). Most articles I find cite purchasing it from Sigma Aldrich, but they sold their last stock in 2013. MilliporeSigma has isotactic...

10 June 2019 6,852 6 View

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08 January 2019 3,512 5 View

How do you construct a crRNA for pCas9 (addgene 42876)?

Hello, I'm designing a CRISPR/Cas9 protocol for school purposes (I attend a extra project next to my study). I have to use the pCas9 plasmid (addgene #42876) for CRISPR eventually. This plasmid...

08 July 2018 5,461 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

In methylene blue test Why is UV absorbance reading very low as soon adsorbent is added and then increasing before reaching equilibrium?

Hello, I'm working on removal of methylene blue. 01. in the dark test very first reading is taken 15 minutes after adding adsorbent to the dye solution and that UV absorbance reading is very...

19 February 2021 472 5 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

What is the UV-vis spectroscopy and HPLC UV detector?

I did spectrum scan to levothyroxine sodium which show highest absorption at wave length=230nm which the absorption =0.136 and it's absorption at 225nm =0.047 but when Injecting it on HPLC...

09 February 2021 1,631 3 View

Please how can i write a proposal on the methodolgies of AAS to determine heavy metalsin water?

Please I want to write a proposal on the methodologies of AAS to determine heavy metals in water. please any link can help.

08 February 2021 3,587 7 View