What is the effect on the conductivity of the thin film during the process of thermal evaporation ?

Why thermally evaporated (VCM 600 V1 configuration of the thermal evaporator) CdTe film found n-type not p-type conductivity whereas all CdTe films deposited by varying the source to substrate...

23 November 2020 7,427 3 View

Can I analyse NGS DATA from an EXCEL spreadsheet?

Hi, I have Fold expression of RNAseq data obtained from Ion Proton sequencer and got the fold change data with mean in an excel sheet. now can I analyse this fold change data using any...

20 October 2020 2,240 3 View

Monolitic construction of brick masonary and concrete simultaniously have any effect ?

Concrete column casting after masonary completed monolithic.

17 August 2020 7,172 3 View

Rentraint Corner of building if need extra stitching diagonal reinforcement ?

If temperature reinforcement already provided ,at reentraint corner need extra diagonal reinforcement ,if needed then what amount of steel.

12 August 2020 1,707 2 View

No title

26 July 2020 1,511 0 View

How to calculate Time Average data (WSS, Velocity, Pressure) in ANSYS Fluent ?

I am doing a blood flow research for which Time average data of Velocity and Wall shear stress is important. The study is transient and my flow time is 0.5 sec. I save data with time step size of...

07 July 2020 4,323 2 View

What is the best management strategy for Diamond Back Moth (DBM) in cole crops?

What should be the best managemnt option for suppressing the DBM problem that farmer can take into consideration?

11 May 2020 4,280 1 View

Is it a good idea to retract a submitted article as the journal is taking too much time to make a decision?

I had submitted a research article related to the field of mechanical engineering (CFD) on the last December, 2019 in a Scopus Indexed journal. It has been around 6 months but the journal...

11 May 2020 9,286 8 View

Where can I get details of SARS-CoV-2 infected patients?

I have been working on peptide vaccine for SARS-CoV-2 or COVID-19 and also recently published some analysis on mutational changes in this virus. It would have been really helpful if I could get...

05 May 2020 2,749 9 View

How does the chemical etching (using HF) affect the temperature sensitivity of a fiber Bragg grating (FBG) inscribed in silica fiber?

Due to chemical etching, the cladding diameter of the silica fibre decreases with time. How does this reduced cladding diameter affect the temperature sensitivity of an FBG, which is inscribed on...

17 April 2020 4,771 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Could not amplify CD28 and CD3z ectodomain from cDNA of Jurkat Cells ?

For my experiments, I have to express ectodomains of human CD28 and CD3z. As a source, I extracted RNA from Jurkat cells and verified the quality of RNA on the gel. I, then, prepared cDNA using...

23 February 2021 7,051 4 View

What effect does the large temperature difference between the two primers have on PCR, including real time PCR?

What effect does the large temperature difference between the two primers have on PCR, including real time PCR? If I did not get a result from real time PCR, is it possible that my problems are...

21 February 2021 4,094 7 View

Why is my qPCR suddently not working?

I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...

21 February 2021 3,437 3 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View