What is the best agar to use to isolate Pseudomonas putida?

More Tengku Zalia Sofea Tengku Zainal Azman's questions See All

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Alex Ignatov

Tengku Zalia Sofea Tengku Zainal Azman According to the original sources, Cetrimide agar agar is King'sB + сetrimide. Although, some other basic recipes for nutrient agar + cetrimide were tested as well (Brown VI, Lowbury EJ. Use of an improved cetrimide agar medium and other culture methods for Pseudomonas aeruginosa. J Clin Pathol. 1965 Nov;18(6):752-6. doi: 10.1136/jcp.18.6.752). So, King's B is just non-selective variant of cetrimide agar.

Tengku Zalia Sofea Tengku Zainal Azman

Alex Ignatov Thank you so much for the help

Phil Geis

General culture information. https://microbenotes.com/pseudomonas-putida/

It will still be difficult to differentiate putida from other Gram Pseudomonas spp. Cetrimide was developed for clinical isolates - some Pseudomonas spp. may not grow..