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Questions related to Molecular Methods
I got a sample of wood chips. After the extraction I found a huge number of nematodes, impossible to distiguish all of them morphologically on species level in a appropriate amount of time with...
01 January 2018 9,627 6 View
I want to use SSR marker to detect nuclear migration in Buller phenomenon in Agaricus bisporus but I want to use another method in my work (e.g. morphological and another molecular method except...
12 December 2017 6,122 3 View
I would like to know the easiest method for confirming ectomycorrhiza presence on grapevine roots?
11 November 2017 6,604 4 View
I am working with a G-protein coupled receptor which is coupled with both Gq and Gi subunits (possibly with other subunits as well). I want to evaluate which subunit(s) is/are getting activated in...
09 September 2017 4,697 2 View
I have used TRV vector for VIGS and PDS homologous gene from soybean.Unfortunately, I did not get the albino phenotype in my soybean plants. I have got yellow phenotype and my qRT-PCR also did not...
20 August 2017 8,986 1 View
08 August 2017 993 1 View
blood parasites
07 July 2017 7,680 3 View
Looking any information whether there is rapid point of care molecular methods to screen/diagnose cervical cancer associated with HPV infection.
30 June 2017 3,141 30 View
06 June 2017 5,506 31 View
Is the study of the origin of a particular flora the same as the study of its affinities? in other words, what is the difference between the term of origin and the term of affinity? in terms of...
01 January 2017 5,082 0 View
What nomenclature prevails for a microorganism (bacteria) if it is taxonomically classified by biochemical methods in a well-defined specie, but when the molecular method (eg 16S) is applied it is...
11 November 2016 9,718 3 View
What are the benefits of Tempo and mini Vidas over cultural based methods and can we prefer mentioned Equipment over Maltitof and other molecular methods if we have to analyze limited number of...
07 July 2016 4,378 2 View
whats the difference with ITS region?
06 June 2016 2,889 3 View
I wanted to know about detection tests beside API or molecular techniques which can differentiate lactobacilli species
05 May 2016 3,477 8 View
Briefly, I am looking to test if there is some pathogenicity caused by (endophytic) bacteria residing within plant tissues. What methods one can adopt to test it in situ? Moreover, please comment...
12 April 2016 9,512 1 View
Hi, I used BsiWi to digest at 55oC for ~4h, but get smears on my agarose gel. It looks like BsiWi didn't properly cut my plasmids. Since heat-inactivation is at 65oC, I was wondering how crucial...
04 April 2016 3,221 3 View
04 April 2016 4,629 1 View
We need the details of RLFP or ARISA methods which can be used to research genes responsibles of proteins production.
03 March 2016 7,337 0 View
I would like to identify fungi (mostly Penicillium and Aspergillus) by molecular methods. I used two pair of primers ITS1 and ITS4, and beta tubulin 2a and beta tubulin 2b. After sequencing I...
03 March 2016 2,163 9 View
And by what morphological test or phenotypically media culture can I do that ? That if I can separated or dislodge plasmids out bacterial cell in any simple technique or with molecular methods.
03 March 2016 6,669 2 View
Currently, my lab is using both ITS regions(ITS1 &ITS2) and RAPD as molecular markers.
27 January 2016 432 3 View
I am working on a de novo assembly for an organism with a fairly small genome (~200 Mb), and for which we have already generated paired end (2x100) Illumina reads which assembled gives an N50...
05 January 2016 483 4 View
01 January 2016 1,374 3 View
When we try to purify phage DNA, we used a host bacteria, how do we differentiate that corresponds to the phage DNA itself and not the bacteria? Which molecular method could be used because i do...
01 January 2016 7,525 3 View