I am working with a G-protein coupled receptor which is coupled with both Gq and Gi subunits (possibly with other subunits as well).

I want to evaluate which subunit(s) is/are getting activated in response to ligand binding. Is there any method by which I can simultaneously track possible subunit(s) activation?

I am aware about cAMP estimation for Go/i subunit functioning and Ca2+ imaging for Gq subunit activity, but I am interested to know if there is any method to simultaneously track the activity. 

I would like to do the estimation either in rat blood vessels or cultured vascular endothelial/smooth muscle cells.

Thanks,

Amreen