I want to use SSR marker to detect nuclear migration in Buller phenomenon in Agaricus bisporus but I want to use another method in my work (e.g. morphological and another molecular method except SSR marker ).
Article Evidence for Outcrossing via the Buller Phenomenon in a Subs...
Any co-dominant marker can be used
CAPS markers are easy to develop. Three markers linked to MAT are interesting: (1) PR6 used in the paper cited above by Aydin Hassanzadeh, (2) markers based on polymorphism in RPB2 (also linked to MAT), or (3) in gene mip (tightly linked to MAT). For the latter, the couple of primers used for A. subrufescens (marker mip) in the paper indicated below has been also successfully used with A. bisporus.
Evidence for amphithallism and broad geographical hybridization potential among Agaricus subrufescens isolates from Brazil, France and Thailand. Fungal Biology 118:1013-1023, DOI 10.1016/j.funbio.2014.10.004
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