And by what morphological test or phenotypically media culture can I do that ?
That if I can separated or dislodge plasmids out bacterial cell in any simple technique or with molecular methods.
Ahmed Elymani
Dear Mohammed,
Your protocol including tests and media ...etc depends on which gene you would like to investigate and what is your research question.
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May Al-Doori
Firstly..You needed to assay the anti-biogram of the microbe(s) you are assaying ;then to distinguish through application of transfer of the antibiotic resistance to check whether its chromosomally located or plasmid-born located genes.You could distinguish that through the frequency of transfer of the antibiotic(s) under study.
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