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Questions related to Molecular Methods
I am working on a de novo assembly for an organism with a fairly small genome (~200 Mb), and for which we have already generated paired end (2x100) Illumina reads which assembled gives an N50...
01 January 2016 7,612 5 View
I need to begin the study of protozoa communities in polluted and non polluted river but is frequently the use of morphological tools, but to my PhD research I need to analise the variation in...
09 September 2015 9,834 3 View
is there any method for phosphate buffer saline plant DNA Eextraction
07 July 2015 6,844 2 View
Biochemical and Molecular methods please and If any a rapid test from forensic sector.
13 May 2015 6,694 3 View
05 May 2015 7,000 4 View
How does it differ from biochemical methods
05 May 2015 7,271 2 View
Tell about the most reliable molecular method.
05 May 2015 4,721 5 View
I am screening the clones obtained after successfully transecting the targeting vector into ES cells. After neomycin selection, the cells were expanded and Now, I am planing for screening to...
09 April 2015 8,410 7 View
04 April 2015 4,782 9 View
I was performing a QIAGEN miniprep and RNase A was not added. Is there a way to correct for this?
04 April 2015 6,687 1 View
Suppose we have located a specific gene on its related chromosome and will subsequently want to determine the inheritance of this gene before crossing.
02 February 2015 4,234 8 View
Vegetative compatibility assay in fungi is a time consuming process for huge numbers of isolates, so is there any alternative molecular method for these assays? I think the best method may be use...
02 February 2015 3,487 2 View
It is known A. baumannii complex is implicated in HAI especially among intensive care patients. Typing the bacteria do help in proper infection control implementation.
17 January 2015 2,830 6 View
01 January 2015 9,413 7 View
In particular, molecular methods on rumen microorganisms to reduce methane
12 December 2014 3,499 1 View
12 December 2014 3,276 0 View
Today, soil microbiologists are able to to quantify the diversity of soil microorganisms and use molecular methods such as oligonucleotide fingerprint groupings (OFRG), rRNA intergenic spacer...
29 November 2014 4,136 3 View
I am doing the research about endotoxin and exotoxin detection, is there any molecular method?
11 November 2014 8,187 4 View
11 November 2014 3,264 3 View
Could someone help me with a biochemical method to determine hydroxylases ,I only find molecular methods , and need to measure enzyme activity. best regards Adriana
10 October 2014 535 2 View
Can anyone suggest how to detect E coli without using any molecular methods in less than 4-5 hrs? I am trying to differentiate both pathogenic and non pathogenic E.Coli species.
08 August 2014 4,423 4 View
I try to detect with microscopy method and now want to confirm with molecular methods.
08 August 2014 2,762 2 View
We have to keep on mind that, the finished product of FISH method is a polycarbonate filter containing bacteria coated with both probe and a marker DAPI (total number of bacteria - We have used...
30 July 2014 527 1 View
I am having problems with my protocol. I am trying to optimize a protocol to assess mitochondrial function in gastric mucosa using the Oroboros. Any tips?
14 July 2014 8,865 5 View