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Questions related to Molecular Methods
In a PCR After the initialistion step at 95 degrees for 1-2 mins, in the second round my guide kept for 15s again at 95 degrees. I know that it is a step in the thermocycle. But why did he keep...
07 July 2014 7,591 16 View
I am having problems with my protocol. I am trying to optimize a protocol to assess mitochondrial function in gastric mucosa using the Oroboros. Any tips?
07 July 2014 6,365 6 View
Newly discovered hormones or markers, that have relation with fertility and some glands cancers.
05 May 2014 7,539 10 View
I need help with my IP protocol. I'm trying to elute with glycine 0.1 M at pH 3. I have used different antibodies immobilized (crosslinked) to magnetic beads. I'm using 10 volumes of glycine...
24 April 2014 1,818 10 View
04 April 2014 716 11 View
T4 DNA ligase efficiency
04 April 2014 7,649 1 View
I did hemolysis with my peptide at different concentration and it was observed that at high concentration the hemolysis was suppressed as compared to low concentration. I am not getting any...
04 April 2014 7,629 0 View
Can anyone suggest a best method to prepare lamina with polypropylene and natural fibers? Where are these protocols available?
04 April 2014 7,887 0 View
Total bacterial counts and immunological or molecular methods.
14 March 2014 5,331 3 View
03 March 2014 9,431 4 View
I want to clone my gene to expreesion vector in Ecoli, after I clean my pcr product, I have a good band. In digestion step, after digesting the gene I don't get any band, but I perform a ligation...
02 February 2014 5,361 17 View
01 February 2014 9,811 16 View
I would like to know if anyone knows what happens if the timing of fixation with p-formaldehyde exceed 48 h and if is reversible the excessive hardening which is obtained mainly at the level of...
01 January 2014 2,256 2 View
I am collaborating in an arthropod research program. I am looking for species identification in relation to the ecological perspective whereby a food source (pollen) would be our interest. Is...
12 December 2013 1,964 20 View
04 December 2013 8,185 19 View
I want to know if my culture organism has nitrogenase activity or not.
11 November 2013 6,672 2 View
I want to do growth curve of mat forming Cyanobacteria. Is it possible? If so, how?
09 September 2013 3,280 0 View
I want to obtain axenic culture of Cyanobactreia.
09 September 2013 8,704 3 View
Units of my RE -10,000 units. How much RE is required to digest 10ul of DNA?
09 September 2013 6,555 10 View
I want to do SEM (Scanning electron microscopy) of Cyanobacteria (mat forming and unicellular).
08 August 2013 9,472 3 View
Is it possible for emerin for example to have different binding patterns in cardiac cells then in skeletal cells?
06 June 2013 5,778 0 View
Do you know if there is whole contamination of RNA in ones lab, then what should be done to remove RNA contamination out of the lab? I know about RNase to be used during PCR samples but what about...
11 November 2012 7,952 13 View
I have trouble to extract mRNA from macrophage / Candida coculture, I'm using 5mm acid washed beads in a tissue lyser, followed by classic RNA extraction (with columns). But I couldn't get any...
09 September 2012 390 3 View
Hello My collegous.......... I am asking about Trypanosoma equperidum if their is any one found it and detect it in molecular methods? and How?
01 January 1970 5,993 0 View