Enteric microbes are often identified in drinking water using simple laboratory techniques. What will be the importance if one choose to isolate these microbes and further suject them to PCR or any other molecular method?
It depends on what is the end-point of your study. The contamination of drinkable water sources or drinkable water delivery systems is of great importance for public health and communitary hygiene.
More advanced methods such as molecular techniques could enable the researcher to screen for certain bacterial strains which are of importance regarding health safety and epidemiologic control. Also, microbial genes for antibiotic resistance could be identified, as well as their dynamics with respect to geographic location, time of day, seasonal variability etc. If a certain source of contamination is suspected to pose a significant health hazard for local or remote areas/agriculture/etc, sample prelevation from both sites (suspected contamination source and local/remote area) and advanced molecular techniques could, in theory establish whether the 2 events are related.
The significance is most likely related to the the higher level of specificity and sensitivity at even low levels of parasitemia or pathogen load, of molecular Diagnostic methods.
Molecular methods can offer avenue for strain-specific diagnosis and supports accuracy of diagnosis.
Invariably, molecular methods support Public health in areas of helping to control transmission following accurate detection at high specificity, support epidemiological survey and molecular characterization of genotype of the pathogen.
Nice and insightful question from you.
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