How can I distinguish S. cerevisiae var. boulardii from other S. cerevisiae strains (preferrably by molecular methods)?

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At what time points should pro-inflammatory cytokines levels be tested in a mouse model of Prototheca algae infection?

I'm working on establishing an murine infection model of the Prototheca algae. Not much is known about the dynamics of the infection, nor the induced immune response. I'd like to use the...

19 October 2020 2,908 2 View

Why I observe the Si peak after analyzing PEO on EDX?

After putting the PEO coated glass substrate into the oven and subjecting it to a temperature of 180 degrees, it obtains a haze on the glass. A peak Si was observed after EDX analysis. Is there...

24 September 2020 6,335 6 View

Which reference gene would you recommend for qPCR analysis in mouse macrophages and epithelial cells?

Which gene would you recommend as reference in those types of cells? Until now, I was working with HPRT but it's not efficient for me anymore. Thank you for all of the answers!

22 September 2020 615 4 View

Meaning of first derivative dM/dH around magnetization reversal in magnetic nanoparticle systems?

When I calculate first derivative of M versus H curve measured for an ensemble of FM nanoparticles (below they blocking temperature) I obtain a very clear maximum, very close to the so called...

04 May 2020 5,116 4 View

IHC Andy Algorithm, how to use it?

Hello everyone! Probably, this issue was already raised, but I would really appreciate online help from someone who didi it. So - could anyone help me with fiji, Andy's algorithm? I try to run it...

26 January 2020 2,249 1 View

Can Alltech OA 1000 Organic acids column be used to analyse solutions in solvents such as DMSO, THF, butanol or ethanol?

I would like to study the dehydration of carbohydrates to HMF in other solvents than water, however I am not too sure if the HPLC column that we have is appropriate for analysing this type of...

03 April 2019 4,022 5 View

How to obtain correctly DC demagnetisation curve (for nanoparticles)?

How can I obtain correctly DC demagnetisation curve (for nanoparticles)? How should I specifically perform the measurements: a) Do I bring to saturation my sample once and then apply increasingly...

20 January 2019 8,441 3 View

How I know that structure is porous from XRD measurement?

In XRD results for porous boron-carbon samples I've got a huge signal (11 000 arb. units) for small angle at 2theta=2.7. I know that this signal can indicate the preaense of small particles or...

14 July 2018 9,366 15 View

What is proper gating strategy for LIVEDEAD stained population in order to exclude dead cells from analysis?

Hi! I would like to discriminate dead cells from my population using LIVEDEAD fixable dye. In principle it stains amine residues in both live and dead cells, but because of cell membrane...

28 October 2017 5,109 3 View

1. Faint multiplex PCR products 2. Internal control of PCR

I usually do my PCR with three primer pairs (pan-dermatophytes, pan-candida, and S.brevicaulis), I've noticed most of products of pan-dermatophytes PCR are very faint. I use almost the highest...

10 July 2017 5,250 4 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein?

Hi In Molecular Dynamics Simulation, how do I make changes to the force field according to my protein? I got this error in the add-ion stage: Fatal error "./ amber99sb-ildn-HHH.ff / force...

01 March 2021 8,747 4 View