I am looking to immortalize skeletal and cardiac myoblasts without messing with p53. Skeletal myoblasts can be readily transformed with a combination of mTERT and CDK4 (I suspect that this will hold true for cardiac myoblasts too). I have plasmids encoding hTERT and hCDK4 on hand. My question is whether the human variants will work in a mouse system. Uniprot gives 94.7% sequence identity between human and murine CDK4, and 62.0% identity between TERTs.
It even works with bovine and pig cells. As shown in:
http://web.expasy.org/cgi-bin/cellosaurus/search?input=24589663
where they have used hTERT and hCDK4.
Curiously I could not find an example in mouse, but there is no reason to think it would not work.
Good luck
Amos
It may depend of the efficiency of transfection. Theoretically, it should work.
Julius R
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