To prevent compound (only 1.5 mg) from degrading, is it better to store in solid form or solution form?

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Any Green Solvents to dissolve PET near room temperature?

I need a PET + Solvent mixture to feed it to a fixed bed reactor for steam reforming.

30 June 2024 2,122 2 View

How to dry peo+pc+liclo4 solution?

how to dry peo+pc+liclo4 solution?

15 February 2024 2,932 0 View

At what conditions PEO polymer is soluble in acetone?

at what conditions PEO polymer is soluble in acetone?

04 February 2024 7,283 2 View

Can dead cells observed after drug treatment interfere with Alamar Blue assay in 96 well plate?

Cytotoxicity study of anticancer compounds using Alamar blue on Cal27 cell lines

31 August 2021 1,105 2 View

What is a good website to check for target specificity of degenerate primers?

Dear all, I have found certain primer pair with degenerate bases (including 'I') in them. These primer pairs are hope to target conserved sequences in of bacterial DNA in a mix of plant and...

17 September 2020 4,237 3 View

How to store TB sputum sample without processing for long term?

We need to store TB sputum sample for cytokine estimation in future. So, for this purpose we need to know about this.

21 February 2017 149 2 View

Evaporation of ACN(Acetonitrile)

I am trying to evaporate my solvent from peo+liclo4 but it is not drying ? any suggestions?

01 January 1970 1,931 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

How are clinical and environmental yeast/fungal strains stored usually in mycology laboratories?

I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...

22 July 2024 6,592 3 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

Method of collection of samples of microorganisms from environmets to labortary?

can any one guid me about tractical method for preservation of microorganism same as bacteria, cyanobacteria and algea, in sampling from environment to Laboratory: 1- Sample collection 2-...

17 July 2024 3,687 11 View