Good afternoon:
I need a recommendation from someone who is preparing a CBB G-250 solution in MIlli Q-water, which can be used as a dye in protein hydrophobicity determination.
We have an issue that once we prepare the solution, its colour is not stable with time until we finish the analysis. With time, its colour starts to be darker.
So, should we prepare and keep the dye in a dark bottle to protect it from light? Is there a possibility the dye powder didn't dissolve completely, and we need to heat the solution during the preparation to ensure the dye's well-dissolving?
Any help or recommendation, please.
Thanks
Warming the solution to help the dye dissolve while stirring it should help to reduce the amount of undissolved dye. If there is still some undissolved dye in the solution, you can remove it by passing the solution through a filter membrane, such as an 0.45 micron filter, using a vacuum flask. It may clog if there is a lot of solid, so you may need two filters to get it all through.
@Adam B Shapiro, thanks for your answer. What do you think for how long should be the stirring ? Also, after dissolving and filtrating. How should be store within the analysis time? Room temperature? In dark bottle or protected from light?
To keep it's color non chsngable until we finish the analysis.
Thanks in advance
If you have the time, consider stirring the solution overnight to make sure as much dye dissolves as possible. If the solution is optically opaque, it probably doesn't have to be protected from light, but it couldn't hurt to wrap the container in aluminum foil. If the pH is close to neutral, there is the risk of bacteria growing in it, so it might be a good precaution to keep it in the refrigerator unless you know it is sterile.
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