Considering the manufacturer did not supply it, I need to equilibrate the column for my western blot experiment.
Use protein elution buffer
Hi How can this equation Ln(LA) = 1.038 + 0.89 ln(X) be applied to calculate the leaf area of a tomato? Can you explain with an example and what is the substitution of Ln and ln?
06 August 2024 2,508 2 View
Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...
26 July 2024 3,460 2 View
In molecular dynamics simulation, to get FEL analysis, I got an error. My Python version is 3.10.7. My input files are made with a lower version of Python. But the final command to generate the...
23 July 2024 5,646 2 View
Hi everybody, We are trying to write a systematic review meta-analysis paper. But I could find 19 references. I think 19 references are not enough to do a meta-analysis section and it is better to...
10 July 2024 5,490 5 View
Dear All, I want to cut PDMS sheet with thickness of 0.8 mm to rectangular shape with Graphtec cutter plotter CE6000-40. What condition do you offer? Do you offer any kind of special speed or force?
25 June 2024 8,111 1 View
I recently acquired a used Illumina HiSeq 2500 and am in need of flow cells compatible with this machine. As I am relatively new to this aspect, I would greatly appreciate any recommendations on...
22 June 2024 5,992 1 View
Dear researchers, Greetings. We have subjected pectin solution to ultrasound to produce oligosaccharides. Initially, as the ultrasound time was increased, the molecular weight decreased....
13 June 2024 881 3 View
Hello every body I use CTAB method for Plant DNA extraction and I need DNA for downstream steps. Unfortunately I have problem in my plant DNA extraction, quality and quantity not as expected, and...
10 June 2024 6,610 2 View
I would be pleased if you could share the report "Animal feed probiotic bacteria market for aquatic animals, BY REGION, 2022-2032" with me.
03 June 2024 255 5 View
I'm a student in zanjan university and I'm writing a proposal but I need quick help. thanks for your help.
02 June 2024 8,950 1 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
I am trying to recover liquid CO2 from a mixture of 0.6 CO2, 0.3 N2, and 0.1 O2. My aim is to recover about 99% liquid CO2 at the bottom of the column and make sure the amount of gaseous CO2...
06 August 2024 4,611 0 View