Does anyone know how to perform whole-mount in situ hybridization on de-embedded OCT samples?

27 November 2024 0 6K Report

I would like to perform whole-mount in situ hybridization on fixed tissues that have been embedded in OCT compound and stored at -80°C.

Has anyone here done this procedure before? And if possible, are there any changes to the coloring procedure for whole-mount in situ hybridization?

The samples were fixed in 4% PFA, embedded in OCT after sucrose substitution following standard protocol, and are currently stored at -80°C.

I would be grateful for any help or advice.

Thank you in advance.

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