Hi all,

I'm working with EDTA blood from cats and I'm having great difficulty achieving lysis with the commercial solutions (BD FACS , Pharma) Lysing solutions at a ratio of 2mL to 100 mcl of blood.

I am incubating for 20 minutes in the dark after vigorous vortexing. After incubation and centrifugation, I obtain a sedimented pellet that cannot be acquired on the cytometer.

I've tried dozens of different methods without success.

Any tips or advice?

Thank you