I’ve got gene expression data from qPCR, and I’m looking to identify which biological pathways are activated or suppressed in my cell line. I’ve already sorted out the fold change values and p-values, but I’m wondering what bioinformatics tools or platforms would be best for this type of analysis.
Thank you for your response 🙏 you have highlighted some very important points. However, is there no application similar to IPA or a tool that can help analyse pathways using qPCR data? The reason I am looking for such a tool is that I am conducting active compound experiments and want to understand how the changes in gene expression caused by these treatments may influence specific pathways. This information would help me generate hypotheses and guide further experiments.