Hi everyone,
Last year, I used Essential 8 media (Gibco) with Rock inhibitor (Stemcells Technology) for the first time to recover my clones, and 6/8 of my clones were recovered gradually (forming large colonies) after 2-3 weeks.
However, this year I have tried to revive two other precious replica using mTeSR-E8 (Stemcells Technology) with Rocki. The cell viability was very low to null.
Would you be able to share your advice on the two media in regards to frozen cell recovery? Do cells have a stock life in -80C? (As mine was stored in -80C for about three months max).
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