I am using RPMI right now but I think DMEM will be better.
I confirm: DMEM is better for FBL culture.
I wonder if anybody knows where I could find and download some sector scan ultrasound images? I need them for doing my homework.
07 April 2024 1,764 1 View
Recently, I was working on cell biology of spinal ligement tissues, the human samples obtained from spinal surgery were easy to identify, but how can I make sure of the isolated spinal tissues...
02 April 2024 2,674 0 View
I mapped in Arcgis, where green is the study species and red is the non-study species, how do I analyse the degree of mixing and the angular scale of the species?
12 March 2024 2,549 1 View
I am trying to optimize my control titration for ITC so that I can ensure my ligand to protein traces are reliable. My small molecule is stored in DMSO. I have been very careful to avoid buffer...
14 February 2024 9,147 3 View
Hello, I have recently switched from doing ligand into protein for ITC because my ligand is in DMSO and has low solubility at 10% DMSO; therefore, now I am doing protein into ligand -- the heat...
09 February 2024 4,680 1 View
Hello, I am having an ITC issue because I cant increase my ligand concentration due to solubility issues. I know there is past literature on reverse titrations being acceptable. I am more asking...
06 February 2024 5,564 2 View
Hello, I am doing ITC and getting repeatable curves that look very promising. After reading some articles, it seems the biggest challenge to avoid is buffer mismatch. Is there a way to rule out...
18 January 2024 1,224 5 View
Hello, I have had moderate success with ITC but most of my issues have been with the Nano Program in fitting. I have a clear curve but the program is not fitting it correctly, does anyone have a...
22 December 2023 9,331 1 View
Any suggestion on microarrays (protein/ DNA/ RNA) that measure enzymes in the phospholipid metabolism pathways? Thank you.
18 December 2023 1,366 3 View
The controlling factors of surface atmospheric pressure are obviously temperature and water vapor pressure. The relationship between atmospheric pressure and temperature is very clear, it is the...
14 December 2023 4,933 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.
02 August 2024 1,323 8 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...
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