I am using RPMI right now but I think DMEM will be better.
I confirm: DMEM is better for FBL culture.
I'm looking for standard axial compressor blade row data files for CFX simulation , so could somebody tell me where can I find them ? Like rotor37 , PBS rotor , and something else about them...
13 February 2021 7,745 3 View
I have a dataset with 96 individuals and 1496 transcripts of interest. I have analyzed the experiment by multiway ANOVA in the Permanova platform of Primer-E. I am satisfied that I have figured...
13 February 2021 7,217 4 View
I want to do LSV ,CV test of ORR(Oxygen reduction reaction). But our laboratory don't have the RDE and RRDE electode. And I was wondering if I can do the tests by magnetic stiring or mechanical...
14 January 2021 1,869 2 View
I am looking for an anti-rat CLEC-2 antibody for IHC in rat lungs. Has anyone come across one? There are a few mouse ones but the data sheets don't say they cross react with rat, and the ones that...
06 December 2020 3,511 0 View
Hello, I have some data that looks like the line on the right, but I'm not sure what function to use. It seems like an exponential added to a line, or something of the like. I think it may be the...
18 November 2020 2,357 3 View
It is my understanding that single-turnover reactions isolate the chemical step, as opposed to being comprised of the chemical step + product dissociation as in kcat. Here's my issue though....
13 November 2020 2,494 7 View
Does anyone know of a method or approach in SPSS to contrast singular mean scores of overall samples, based only on the overall mean value, standard deviation figure and sample size? The data is...
29 September 2020 5,872 6 View
I am performing a TSA to investigate binding of a ligand to the protein by performing a serial dilution of ligand against same conc. of protein (~2uM). Interestingly at low ligand conc the...
02 August 2020 3,167 1 View
While I'm working on a project about landslides on the Tibet Plateau, I encountered many large and valley-filling deposits that seem to be some kind of mass movement but cannot find their source...
30 July 2020 6,134 5 View
09 July 2020 9,914 1 View
02 March 2021 3,060 3 View
28 February 2021 9,936 2 View
After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .
28 February 2021 7,738 3 View
I want to use 0.1% gelatin. Should that be made in PBS or sterile water? Should I autoclave the gelatin soln after making it in water/PBS?
28 February 2021 274 2 View
Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...
27 February 2021 3,904 2 View
As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...
27 February 2021 2,595 8 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...
26 February 2021 6,151 2 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines
24 February 2021 2,268 3 View
