I am using RPMI right now but I think DMEM will be better.
I confirm: DMEM is better for FBL culture.
I am currently working on measuring intracellular calcium levels when they are treated with Bapta-AM. I plated the cells at 40,000 cell/well/100 uL overnight in a 96 well plate and then loaded...
05 June 2017 438 2 View
I was wondering what the limiting factors to pseudo-typing viruses in respect to treating cancer and selectively killing cells that need to be disposed of. A brief overview on how pseudo-typing...
04 May 2017 1,167 2 View
I am wondering if senescent cells can be transfected by gamma retroviruses in order to turn on or turn off genes.
01 January 1970 1,788 3 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
i am facing the difficulty to find the right and latest topic for my thesis. i searched and read hundreds of articles but i couldn't find the right one. what can i do I'm really confused.
02 August 2024 1,323 8 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
Hello, Dear friends, I am surveying as part of my thesis research and would greatly appreciate your participation. The study investigates how social media influences dental health behaviors...
01 August 2024 9,349 2 View
SMEs are backbone of the country's white economy, most of them are II generation entrepreneurs and migrated from family owned business policies, and having sufficient higher educations for...
01 August 2024 5,562 2 View
