Hello everyone.
i want to isolate and culture bacteria from infected non vital teeth. predominantly the anaerobes are responsible for the primary infection.
my question is can i use BHI media for transporting the sample to lab and for incubation in lab?
after incubation aerobic + anaerobic in BHI for 24 and 48 hr respectively the samples will be further processed on enriched media.
Mcconkey , chocoalte and blood agar.
Murat Aydin
Dear Hasan
If you modify BHI broth by adding cysteine (0.4 g/L), hemin (5mg/L) then you do not loss fastidious anaerobes.
Good luck
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