Dear all,

I am wondering how to evaluate the purity of monocyte derived macrophages (MdM) using flow cytometer. MdM are obtained by adherence isolation, through culture of purified CD14+ monocytes at 2 × 106 cells/mL in macrophage media (RPMI-1640 with 10% FCS, 5% human AB serum, 1% HEPES (10 mM), 1% P/S, and 10 ng/mL MCSF) for 3 days, washing and culturing another 3–5 days. How to differentiate at morphological level (using optic microscope) between CD14+ monocytes and MdM? May anyone send me links or articles about this topic? Thanks in advance.