hi, all. I am facing problem in the calculation to prepare minimal media (composition below) that contain 10 ppm chromium. I want to prepare 100 ml minimal media contained 10 ppm of chromium. can anyone help me to do that?
composition of minimal media
solution A
KH2PO4 0.3gr
Na2HPO4 0.6gr
NH4Cl 0.2gr
NaCl 0.5gr
DI water ~80ml
solution B
glucose 0.8gr
MgSO4.7H2O 0.01gr
agar 1.5gr
Di water ~20ml
if I add 10 ppm chromium in solution A, is my media already contain 10 ppm of chromium?
thanks in advance.
Dear Adisya Prima,
It doesnot matter whether you add 10 ppm of chromium in solution A or solution B. Totally the solution should contain 10 ppm. It is better to add 1 mg of chromium in solution A or solution B. Because when you mix it, the chromium concentration will be 1mg/100 ml which is equivalent to 10 mg/L. Just take any chromium containing substrate which should not inhibit the growth of the microoranisms in minimal media.
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