Hi All,
Am trying to quantify benzalkonium chloride by LCMSMS. I have optimised the method got the parent and daughter ion. Have injected the standard, peak was eluting. After that injected blank sample found some peaks in the same mass with the same response. Tried with different mobile phase but found the same peak with same mass in blank sample. checked for injector port carryover but injecting directly to loop. but found the same peak eluting. Can anyone help me resolving the same.
If I remember BAC is 'very' hard to clean. See attached (they may have an idea on cleaning);
https://www.thermofisher.com/search/results?query=Benzalkonium%20Chloride%20HPLC&focusarea=Search%20All
See the attached discussion on RG;
https://www.researchgate.net/post/Does_anyone_have_a_method_for_analyzing_Benzalkonium_Chloride_using_HPLC_on_a_C18_Column?view=58c87aa75b49526f043d89dc
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