Immunology

Immunology is a broad branch of biomedical science that covers the study of all aspects of the immune system in all organisms. It deals with the... | Contact experts in Immunology to get answers

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Questions related to Immunology

Why do people do vivo experiments with scopolamine and in vitro experiments with glutamate-induced impairment model?

Why do people do in vivo experiments with scopolamine and in vitro experiments with glutamate-induced impairment model together? Why do not do an in vitro experiment with glutamate, not...

02 November 2018 4,944 1 View

Can anyone help me with T cell differentiation protocol?

Hi, I am trying to do in-vitro T cell differentiation from mouse naive CD4+CD62L+ T cells but there is something wrong that I cant figure out. I tried several times with different plates and...

19 October 2018 585 5 View

Why does T line disappear very fast in the competitive assay?

I performed competitive assay in lateral flow. Ag was conjugated with gold particles and Ab was painted on the membrane. T line showed up right after positive control was loaded, while T line...

10 October 2018 6,103 0 View

For T-Cell Receptors, why is there more focus on the beta than alpha chain?

I apologize but I am still trying to get up to date on the immunology field. All the papers I read are solely focusing on the beta chain of TCRs. Why is this the case? Shouldn't efforts be focused...

10 October 2018 7,743 2 View

Are antigens in an animal's skin responsible for allergic reactions in humans?

Immunology isn't my field of expertise so I'm a little rusty on the jargon, and just want to confirm with someone who knows better than I do. I have just finished reading an old study that...

10 October 2018 9,939 1 View

Does different variations of red/pink TTC dyed colonies show presence of RD (respiration deficient) yeast?

Yeast Y17097( Δgsh1) strain was affected by different strength pulsed electric fields generating oxidative stress to cells and causing cell death or presumably mtDNA mutations. I want to detect...

26 September 2018 7,824 0 View

How to analyze a set of 25 cytokines in 60 samples from the 4T1 cancer mouse model?

We tested a potential anti-cancer treatment in balb/c mice injected with 4T1 cells (25 animals). We collected blood initially, at D7, D14, D21 and D28; We also collected supernatant of spleen...

09 September 2018 2,945 4 View

How to determine antibody titer in mouse serum?

Hi all, I am trying to determine the antibody titer in immunized mouse serum specific to an antigen. I have found people using serial dilutions and determine the titer by the most diluted sample...

09 September 2018 5,625 4 View

Ficoll or Histopaque?

I am going to isolate monocytes from whole blood using either Histopaque or Ficoll combined with MACS. Does any of you have experiences using either Histopaque or Ficoll?

04 September 2018 3,872 5 View

How does Luminex calculate the sensitivity of Magpix CCD?

In the technical specifications for the Magpix instrument, it is noted that the minimum number of Phycoerythrin molecules it can detect per bead is about 700. I am interested in knowing how they...

08 August 2018 7,082 0 View

Quisiera saber como podría conseguir las obras o experimentos escritas/os por veterinarios como Calmette, Claude Bernard, Toulose, entre otros?

Muy buenas tardes! Estoy buscando artículos antiguos de los anos 1800 y 1900 de veterinarios, médicos y entre otros ligados al área de la salud, referente al área de inmunología?

08 August 2018 9,052 0 View

What are some commonly observed problems associated with designing/using virus like particles as vaccines?

For those of you who have used/built VLPs in your research what are some limitations you have observed? What do you think is preventing them from being used universally as vaccines? What are some...

08 August 2018 2,539 5 View

Dear Collegues, Have any amongst you heard about an Immunology conference in Paris 17-19 Sept. 2018 organized by Scientifictree ? Is it real ?

Last year I was invited to join the organizing committee and give a key note speak in Paris 17--19 September this year. I transferred the speakers fee and since then I heard nothing : No...

08 August 2018 8,388 7 View

Mental Health Open Access DATABASE?

Hello everybody,I am looking for open access database available to work on a research concerning mental health. Any advice? Thanks in advance, Fabio

02 August 2018 6,779 1 View

Flow Cytometer Cell Counts ?

I am using flow cytometer and trying to find out the cell concentration. For this purpose I am using CountBright™ Absolute Counting Beads. In its protocol it is saying "at least 1,000 bead events...

31 July 2018 1,909 2 View

Which would be a good combination of antibodies to detect Memory t cells by Immunofluorescence or Multiplexing?

I have been looking into specific markers to detect memory T cells, as there is not just one unique marker to detect them, I was wondering which would be a good combination, would CD45RO/CD3 work?

25 July 2018 1,681 5 View

How to label Ds-Red/GFP cells?

Hello Hello, I am trying to stain DsRed/GFP lymphocytes in recipient tissue. Tissues are frozen spleen embedded in OCT gel. I used a primary Rabbit polyclonal to RFP (Anti-RFP antibody ab62341)...

21 July 2018 8,684 3 View

Best body condition formula for reptiles?

I am currently working on a immunology and parasitology project on crocodiles for my masters, and I'd like to ad another layer to my assignment to make it a more rounded project. I a looking for...

07 July 2018 1,398 0 View

Any 16S Microbial Abundance Network Analysis Suggestions?

I am an undergraduate neuroscience and bioinformatics research assistant and my personal project has been to explore the gut microbiota of an EAE mouse model. There are three treatment groups:...

07 July 2018 454 3 View

Aggressive serous epithelial ovarian cancer is potentially propagated by EpCAM+CD45+ phenotype. ?

hello

07 July 2018 7,350 0 View

How do we obtain MHC peptide binding groove protein sequences?

Hi all, I am trying to obtain the protein sequences of the peptide binding grooves of MHC class I and class II alleles, but am unsure how to go about it. We know that for class I, the peptide...

07 July 2018 6,708 0 View

Antibody hitting its target; based on luck or drawn to it like a magnet is drawn to metal?

I have a question about the dynamics of antibodies. Lets say you have a solution with a fixed antigen and you add antibodies to it, the antibodies are then floating around and some antibodies will...

07 July 2018 8,512 0 View

THP-1 transfection (NEON electroporation system) condition with subsequent recovery and PMA induction ?

Dear all, I have been performing transfection of GFP-tagged plasmids into THP-1 cells using NEON electroporation system. However, the relatively low transfection efficiency suggests that I might...

07 July 2018 1,242 2 View

Naíve CD4+ T cells that don't get activated?

Dear all, I would like to know if any of you is having issues with the Naive CD4+ T Cell Isolation Kit II, human from Miltenyi Biotec or with the CD3/CD28 activation beads for naíve CD4+T cells...

07 July 2018 5,158 9 View

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