09 September 2018 4 6K Report

Hi all,

I am trying to determine the antibody titer in immunized mouse serum specific to an antigen. I have found people using serial dilutions and determine the titer by the most diluted sample that did not show 2X as much signal as background well. I tried to find a protocol that described it in Current Protocol of Immunology but could not.

What is the most trustful way/ mostly recognized way to determine antibody titers?

Thanks!

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