How to do MD simulation of docked protein complex after docking .?

I have perform some protein-protein docking studies. I used Hdock, Prizm. But I am unable to find a proper method to verify my docked structure. I also docked my structure in Hex program to get...

19 October 2020 4,083 7 View

How can we measure the content of happiness in a person by applying various mathematical and soft computing techniques?

Here I just want to know about the actual parameters to measure the content of happiness in a person. With the help of these parameters a neural network can be generated and maintained to achieve...

22 September 2020 7,666 2 View

Which tool to use for Sample Size Calculation studies for AI based studies involving Machine learning or Deep Learning?

I want to know of an online tool or formula to give a vague idea about minimum number of enrolees for a study involving use of machine or deep learning.

01 August 2020 747 3 View

What all we can do to troubleshoot a genotyping PCR?

We have been characterizing our transgenic mice lines as a routine job in lab for years and It has been working fine until a recent batch of primers for VEGF has arrived. We started observing...

28 July 2020 7,639 15 View

What could be the potential reasons for a long DNA molecule not able to attach to the mica surface for AFM imaging even in the presence of Mg++ ?

29 June 2020 2,075 2 View

How Machine Learning Algorithms can be applied for the High Altitude Wetland restoration? Any idea how ML can be integrated with Geospatial Tools??

Wetland Extent Mapping

09 February 2020 3,921 3 View

How to find gene with similar function across Bacteroides and Firmicutes?

My genes interest is EntA in E.coli. I want to find genes with similar function like EntA in any members of Bacteroides and Firmicutes. I need the DNA sequence of EntA-like genes in these phyla.

01 January 2020 2,400 1 View

I am looking for the melting point and heat of fusion of graphene oxide. Could someone please help me in this regard?

Any reference in this regard would be very helpful.

06 December 2018 6,561 3 View

Biofilm Fixation for Confocal?

I have grown biofilm and I need to fix it and stain it. 1. Clean the entire disk carefully and aseptically with pipette so that extra biofilm which are present over the walls. 2. Cells are fixed...

17 July 2018 3,954 5 View

What would be the best for miRNA NGS validation?

I would like to validate miRNA NGS data by qRT-PCR. This theoretically could be done from total RNA samples (the one used for sequencing purpose).What approach should I use to isolate the miRNA or...

25 February 2018 4,574 2 View

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02 March 2021 3,060 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View